Jefferis R, Mageed R A
Department of Immunology, University of Birmingham Medical School, UK.
Scand J Rheumatol Suppl. 1988;75:89-92. doi: 10.3109/03009748809096746.
Monoclonal rheumatoid factors (MRF) of the Wa idiotypic family have been studied for Fc gamma epitope specificity and the expression of idiotopes recognized by murine monoclonal antibodies. The majority of MRF recognized the Ga antigen or a closely similar iso-allotypic epitope expressed on IgG1, 2, 4 and IgG3.G3m(s.t.) proteins. MRF having the iso-allotypic specificity expressed a similar profile of V-region sub-group and idiotypic serological markers. One protein, Kas, whilst recognizing a distinct Fc gamma epitope also expressed these V-region markers and was highly homologous, from primary sequence analysis, with a MRF of Ga specificity. We conclude that these MRF recognize overlapping non-identical epitopes possibly including the same immuno-dominant residue and consequently demonstrate restricted V-gene usage.
已对Wa独特型家族的单克隆类风湿因子（MRF）进行了研究，以确定其Fcγ表位特异性以及鼠单克隆抗体识别的独特型表位的表达情况。大多数MRF识别在IgG1、2、4和IgG3.G3m(s.t.)蛋白上表达的Ga抗原或密切相似的同种异型表位。具有同种异型特异性的MRF表达了相似的V区亚群和独特型血清学标志物谱。一种蛋白Kas，虽然识别一个独特的Fcγ表位，但也表达这些V区标志物，并且从一级序列分析来看，与具有Ga特异性的MRF高度同源。我们得出结论，这些MRF识别重叠但不相同的表位，可能包括相同的免疫优势残基，因此表明V基因的使用受限。
