Fisher H P, Doppl W, Osborn M, Altmannsberger M
Department of Pathology, Justus Liebig University Giessen, Federal Republic of Germany.
Virchows Arch B Cell Pathol Incl Mol Pathol. 1988;56(2):71-6. doi: 10.1007/BF02890004.
A combined hepatocellular-cholangiocarcinoma (CHC) of transitional subtype and the surrounding cirrhotic liver tissue were investigated immunocytochemically by monoclonal antibodies specific for each of the keratin polypeptides 7, 8, 18 and 19. Different keratin subsets were found in different parts of the tumour. The hepatocellular component reveals keratins 8 and 18, with the bordering cells of trabecular formations additionally expressing keratins 7 and 19. The same keratins i.e. 7, 8, 18, 19 were found in normal bile duct epithelium as well as in cholangiocarcinomatous and transitional areas of hepatocellular and cholangiocellular differentiation. Normal hepatocytes express only keratin 8 and 18. In cirrhotic liver some modified hepatocytes additionally express keratin 7. When ductal transformation is observed in the marginal parts of portal tracts and fibrous septa the keratin polypeptide pattern mimics that of bile duct epithelium. The cholangiocellular metaplasia of hepatocytes observed here correlates well with findings in hepato-organogenesis and hepatocarcinogenesis and suggests that the transitional subtype of combined hepatocellular-cholangiocarcinoma is a variant of hepatocellular carcinoma.
采用针对角蛋白多肽7、8、18和19的单克隆抗体，对一例移行亚型的肝细胞-胆管癌（CHC）及其周围的肝硬化肝组织进行免疫细胞化学研究。在肿瘤的不同部位发现了不同的角蛋白亚群。肝细胞成分显示角蛋白8和18，小梁结构的边界细胞还额外表达角蛋白7和19。在正常胆管上皮以及肝细胞和胆管细胞分化的胆管癌和移行区域中发现了相同的角蛋白，即7、8、18、19。正常肝细胞仅表达角蛋白8和18。在肝硬化肝中，一些经过修饰的肝细胞还额外表达角蛋白7。当在门管区和纤维间隔的边缘部分观察到导管化生时，角蛋白多肽模式类似于胆管上皮。此处观察到的肝细胞胆管细胞化生与肝器官发生和肝癌发生的结果密切相关，提示肝细胞-胆管癌的移行亚型是肝细胞癌的一种变体。
