Lovaglio Jamie, Artwohl James E, Ward Christopher J, Diekwisch Thomas Gh, Ito Yoshihiro, Fortman Jeffrey D
Biologic Resources Laboratory, University of Illinois at Chicago, Chicago, Illinois, USA.
Mayo Clinic, Rochester, Maine, USA.
Comp Med. 2014 Apr;64(2):115-20.
Three mice (2 male, 1 female; age, 5 to 16 mo) from a mouse line transgenic for keratin 14 (K14)-driven LacZ expression and on an outbred Crl:CD1(ICR) background, were identified as having distended abdomens and livers that were diffusely enlarged by numerous cysts (diameter, 0.1 to 2.0 cm). Histopathology revealed hepatic cysts lined by biliary type epithelium and mild chronic inflammation, and confirmed the absence of parasites. Among 21 related mice, 5 additional affected mice were identified via laparotomy. Breeding of these 5 mice (after 5 mo of age) did not result in any offspring; the K14 mice with polycystic livers failed to reproduce. Affected male mice had degenerative testicular lesions, and their sperm was immotile. Nonpolycystic K14 control male mice bred well, had no testicular lesions, and had appropriate sperm motility. Genetic analysis did not identify an association of this phenotype with the transgene or insertion site.
来自一个角蛋白14（K14）驱动LacZ表达的转基因小鼠品系、背景为远交Crl:CD1(ICR)的三只小鼠（2只雄性，1只雌性；年龄5至16个月），被发现腹部膨隆，肝脏因大量囊肿（直径0.1至2.0厘米）而弥漫性肿大。组织病理学显示肝囊肿内衬胆管型上皮，伴有轻度慢性炎症，并证实无寄生虫。在21只相关小鼠中，通过剖腹术又发现了5只患病小鼠。这5只小鼠（5月龄后）繁殖未产生任何后代；患有多囊肝的K14小鼠无法繁殖。患病雄性小鼠有睾丸退行性病变，其精子无运动能力。非多囊性K14对照雄性小鼠繁殖良好，无睾丸病变，精子运动能力正常。基因分析未发现该表型与转基因或插入位点存在关联。
