Trafny E A, Grzybowski J
Department of Microbiology, Military Institute of Hygiene and Epidemiology, Warsaw, Poland.
Acta Microbiol Pol. 1988;37(2):191-204.
Lipopolysaccharides of seven Fisher's immunotypes of P. aeruginosa, extracted by water-phenol method, were fractionated on Sepharose 2B column. On the basis of molecular weight sugar and KDO content, eluents were separated into 4 fractions. An analysis of the antigenic specificity of the chromatographic fractions of seven immunotypes of LPS was carried out, using sera of mice vaccinated with several crude LPS preparations or whole-cell suspensions each of P. aeruginosa immunotypes, by ELISA. The antigenic specificity of fraction 1 and 2 of several immunotypes (with the exception of LPS from immunotype 2) in reaction with mice antisera for crude LPS was shown. Not quite full specificity of fractions 1-3 of all LPS preparations during analysis of these fractions reactivity with antisera to whole P. aeruginosa cells were observed, but specific reactions predominated in all test systems except LPS 2.
采用水-酚法提取的7种费氏免疫型铜绿假单胞菌的脂多糖，在琼脂糖2B柱上进行分级分离。根据分子量、糖和KDO含量，洗脱液被分离为4个级分。通过ELISA法，使用接种了几种粗制脂多糖制剂或每种铜绿假单胞菌免疫型全细胞悬液的小鼠血清，对7种免疫型脂多糖色谱级分的抗原特异性进行了分析。结果显示，几种免疫型（免疫型2的脂多糖除外）的级分1和级分2与粗制脂多糖小鼠抗血清反应时具有抗原特异性。在分析这些级分与铜绿假单胞菌全细胞抗血清的反应性时，观察到所有脂多糖制剂的级分1-3的特异性不完全，但除脂多糖2外，在所有测试系统中特异性反应占主导。
