College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, China.
College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, China.
Environ Pollut. 2014 Jun;189:169-75. doi: 10.1016/j.envpol.2014.03.001. Epub 2014 Mar 24.
Polychlorinated biphenyls (PCBs) are thought to cause adverse health effects, particularly endocrine disruption. However, results on the estrogenic activities of a large set of PCB congeners through hormone receptors have not been fully studied. In this study, we evaluated the anti/estrogenic effects of 20 PCBs using an in vitro dual-luciferase reporter gene assay. PCB 18, 28, 49, 52, 99, 101, 103, 110, and 128 exhibited estrogenic effects, whereas PCB 118, 138, 163, 170, 180, 187, 194, 199 and 203 behaved as anti-estrogens. In particular, PCB 30 and 44 exhibited both agonistic and antagonistic activities in the dual-luciferase reporter gene assay. The results obtained from the dual-luciferase reporter gene assay, yeast two-hybrid assay and E-SCREEN were compared, suggesting that the dual-luciferase reporter gene assay is a useful approach for high-throughput screening. We also predicted the possible relationship between the chemical structures and the estrogenic effects of PCBs.
多氯联苯（PCBs）被认为会对健康造成不良影响，特别是会破坏内分泌系统。然而，人们尚未充分研究大量 PCBs 同系物通过激素受体发挥雌激素活性的情况。在这项研究中，我们使用体外双荧光素酶报告基因检测，评估了 20 种 PCBs 的抗雌激素/雌激素效应。PCB 18、28、49、52、99、101、103、110 和 128 具有雌激素效应，而 PCB 118、138、163、170、180、187、194、199 和 203 则表现为抗雌激素。特别是 PCB 30 和 44 在双荧光素酶报告基因检测中表现出激动剂和拮抗剂活性。将双荧光素酶报告基因检测、酵母双杂交检测和 E-SCREEN 的结果进行比较，表明双荧光素酶报告基因检测是一种高通量筛选的有用方法。我们还预测了 PCBs 的化学结构与雌激素效应之间可能存在的关系。
