Bell C D, Shokry P E, Stadler J
Department of Pathology, Central Hospital, Toronto, Ontario, Canada.
Am J Clin Pathol. 1989 Apr;91(4):377-85. doi: 10.1093/ajcp/91.4.377.
Biopsies from 52 cases of infiltrating duct carcinoma of breast were assessed for proportions of tumor infiltrating stroma as "thin" strands and as confluent cell sheets. The proportions of cell nuclei arbitrarily designated "dark" were determined for each whole tumor and for their above-mentioned invasive compartments and were compared with the tumor's nuclear grade, mitotic index, gross size, axillary metastatic status, and hormone receptivity. Well-differentiated tumors tended to have more cells with "dark" nuclei and more of the tumor mass invading the stroma as "thin" strands than poorly differentiated ones. Tumors that had axillary metastases also tended to have high mitotic indices, low proportions of cells with "dark" nuclei (and conversely high proportions of cells with vesicular nuclei) in confluent sheets. Tumors that had positive results for one or both hormone receptors had a greater component of trabecular stromal invasion than tumors with negative results for both hormone receptors that grew as confluent cell sheets. It is suggested that tumor cell nuclear staining "density" might be a clinically useful prognostic index despite the finding that semiquantitative assessments of nuclear density can be modified by the mode of stromal invasion.
对52例乳腺浸润性导管癌的活检标本进行评估，以确定肿瘤浸润基质呈“细”条索状和融合细胞片的比例。针对每个完整肿瘤及其上述浸润成分，确定任意指定为“深色”的细胞核比例，并与肿瘤的核分级、有丝分裂指数、大体大小、腋窝转移状态及激素受体情况进行比较。高分化肿瘤往往比低分化肿瘤有更多细胞核呈“深色”的细胞，且更多肿瘤团块以“细”条索状浸润基质。有腋窝转移的肿瘤在融合片中也往往有高有丝分裂指数、细胞核呈“深色”的细胞比例低（反之泡状核细胞比例高）。一种或两种激素受体检测呈阳性的肿瘤，与两种激素受体均为阴性且呈融合细胞片生长的肿瘤相比，小梁状基质浸润成分更多。尽管发现核密度的半定量评估可因基质浸润方式而改变，但提示肿瘤细胞核染色“密度”可能是一个具有临床意义的预后指标。
