一种针对人睾丸抗苗勒管激素的单克隆抗体。

A monoclonal antibody against human testicular anti-müllerian hormone.

作者信息

Legeai L, Lefèvre G, Vigier B, Picard J Y, Josso N

机构信息

Unité de Recherches sur l'Endocrinologie du Développement, INSERM, Hôpital des Enfants-Malades, Paris, France.

出版信息

Am J Reprod Immunol Microbiol. 1988 Oct;18(2):39-43. doi: 10.1111/j.1600-0897.1988.tb00232.x.

DOI:10.1111/j.1600-0897.1988.tb00232.x

PMID:2467567

Abstract

Using immunochromatography on a polyclonal antibody, testicular anti-Müllerian hormone (AMH) was purified from homogenates of human fetal testicular tissue and used as an antigen for hybridoma production. Two IgM clones were obtained. Both recognized AMH on biopsies of human testicular tissue and one blocked its anti-Müllerian activity. This monoclonal antibody (MAb) exhibited a relatively high affinity for AMH, and was studied further. It is interspecific, recognizing AMH in other mammalian species. The study of this MAb in relation to an IgM MAb raised against bovine AMH (bAMH) indicates that both MAbs recognize different but related epitopes on bAMH.

摘要

利用针对多克隆抗体的免疫层析法，从人胎儿睾丸组织匀浆中纯化出睾丸抗苗勒管激素（AMH），并将其用作制备杂交瘤的抗原。获得了两个IgM克隆。两者均能识别人类睾丸组织活检中的AMH，且其中一个能阻断其抗苗勒管活性。这种单克隆抗体（MAb）对AMH表现出相对较高的亲和力，并进行了进一步研究。它具有种间特异性，能识别其他哺乳动物物种中的AMH。对该MAb与针对牛AMH（bAMH）产生的IgM MAb的相关研究表明，这两种MAb识别bAMH上不同但相关的表位。