牛睾丸抗苗勒管激素的生化分析

Biochemical analysis of bovine testicular anti-Müllerian hormone.

作者信息

Picard J Y, Goulut C, Bourrillon R, Josso N

出版信息

FEBS Lett. 1986 Jan 20;195(1-2):73-6. doi: 10.1016/0014-5793(86)80133-8.

Abstract

Direct biochemical analysis has been applied to bovine testicular anti-Müllerian hormone (AMH), purified from incubation medium of bovine fetal testes by immunochromatography on a monoclonal antibody. The hormone contains a high proportion of hydrophobic amino acids and 13.5% carbohydrate. The oligosaccharide composition suggests that both N- and O-glycosidically linked chains are present. The molecular extinction coefficient is 3.27 +/- 0.06. One RIA unit, defined as the amount of hormone released by 1 g fetal bovine testicular tissue incubated during 4 h, corresponds to 3.06 +/- 0.17 microgram protein.

摘要

直接生化分析已应用于牛睾丸抗苗勒管激素（AMH），该激素通过在单克隆抗体上进行免疫色谱法从牛胎儿睾丸的培养液中纯化得到。该激素含有高比例的疏水氨基酸和13.5%的碳水化合物。寡糖组成表明存在N-糖苷键连接和O-糖苷键连接的链。分子消光系数为3.27±0.06。一个放射免疫分析单位定义为1克胎儿牛睾丸组织在4小时孵育过程中释放的激素量，相当于3.06±0.17微克蛋白质。