Cai Mingbo, Hu Zhenhua, Liu Juanjuan, Gao Jian, Liu Chuan, Liu Dawo, Tan Mingzi, Zhang Danye, Lin Bei
Department of Obstetrics and Gynecology, Shengjing Hospital Affiliated to China Medical University, No. 36 Sanhao Street, Heping District, Shenyang 110004, Liaoning, China.
Int J Mol Sci. 2014 Mar 26;15(4):5292-303. doi: 10.3390/ijms15045292.
Beclin 1 is an autophagy-associated protein involved in apoptosis and drug resistance, as well as various malignancies. We investigated the expression of Beclin 1 protein in ovarian epithelial tissues and correlated it with the prognosis of ovarian cancer. Beclin 1 protein expression was determined using immunohistochemistry in 148 patients with ovarian epithelial cancer, 26 with ovarian borderline tumor, 25 with benign ovarian tumor, and 30 with normal ovarian tissue. The relationships between Beclin 1 protein expression and ovarian cancer pathological characteristics were analyzed. The risk factors for ovarian cancer prognosis were analyzed using Cox's regression model. A survival curve was plotted from the follow-up data of 93 patients with ovarian cancer to analyze the effects of Beclin 1 expression on the prognosis of ovarian cancer. The positive rates of Beclin 1 were significantly higher in ovarian epithelial cancer (148) and borderline tumor (26) than in benign ovarian tumor (25) or normal ovarian tissue (30) (all p<0.001). The surgical stage and Beclin 1 expression were both independent risk factors for ovarian cancer prognosis (both p<0.05). Patients with high Beclin 1 levels showed better survival than those with low Beclin 1 levels (p=0.009). Beclin 1 protein is upregulated in ovarian epithelial cancer and is a prognostic factor of ovarian cancer.
Beclin 1是一种与自噬相关的蛋白质，参与细胞凋亡、耐药性以及多种恶性肿瘤的发生发展。我们研究了Beclin 1蛋白在卵巢上皮组织中的表达情况，并将其与卵巢癌的预后相关联。采用免疫组织化学方法检测了148例卵巢上皮癌患者、26例卵巢交界性肿瘤患者、25例卵巢良性肿瘤患者及30例正常卵巢组织中Beclin 1蛋白的表达。分析了Beclin 1蛋白表达与卵巢癌病理特征之间的关系。采用Cox回归模型分析卵巢癌预后的危险因素。根据93例卵巢癌患者的随访数据绘制生存曲线，分析Beclin 1表达对卵巢癌预后的影响。Beclin 1在卵巢上皮癌（148例）和交界性肿瘤（26例）中的阳性率显著高于卵巢良性肿瘤（25例）或正常卵巢组织（30例）（均p<0.001）。手术分期和Beclin 1表达均为卵巢癌预后的独立危险因素（均p<0.05）。Beclin 1水平高的患者比Beclin 1水平低的患者生存情况更好（p=0.009）。Beclin 1蛋白在卵巢上皮癌中上调，是卵巢癌的一个预后因素。
