Saluja Tajindra Singh, Hosalkar Rashmi
Department of Oral Pathology and Microbiology, Faculty of Dental Sciences, King George's Medical University, Lucknow, Uttar Pradesh, India.
Department of Oral Pathology and Microbiology, MGM Dental College and Hospital, MGM Institute of Health Sciences, Navi Mumbai, Maharashtra, India.
Head Neck Pathol. 2025 Feb 5;19(1):17. doi: 10.1007/s12105-025-01755-5.
Autophagy is involved in several critical cellular processes regulating cell survival and death. Past research suggests that it may either act as a tumor suppressor or promote tumor progression. The purpose of this systematic review and meta-analysis was to evaluate the clinical and prognostic utility of a significant autophagy related protein-Beclin1, in oral squamous cell carcinoma (OSCC).
Preferred Reporting Items for Systematic Review and Meta-Analysis (PRISMA) guidelines were followed. Relevant literature was retrieved from PubMed, ScienceDirect and Google Scholar database. After removal of duplicates quality of the studies was assessed using Newcastle-Ottawa Scale. Heterogeneity was assessed using I index. Random effect model was used if I was more than 50% else fixed effect model was selected. Meta-analysis was carried out using Review Manager (RevMan; Version 5.4).
Five studies with 494 cases were included in this meta-analysis. Beclin1 expression in OSCC was not significantly associated (p > 0.05) with gender, age, tumor size, lymph node metastasis, histological differentiation and overall survival. Nevertheless, a trend for low Beclin1 expression favoring tumor progression was observed. Sensitivity analysis revealed significant nodal positivity related to low Beclin1 expression.
This study provided an overview of Beclin1 expression in OSCC and highlighted additional evaluations while its use as a prognostic marker. It is suggested that future studies should assess both nuclear as well as cytoplasmic expression of Beclin1 and report intra- and inter-tumor variations in its expression relating to clinicopathological parameters.
自噬参与调节细胞存活和死亡的多个关键细胞过程。过去的研究表明，它可能既作为肿瘤抑制因子发挥作用，也可能促进肿瘤进展。本系统评价和荟萃分析的目的是评估一种重要的自噬相关蛋白——Beclin1在口腔鳞状细胞癌（OSCC）中的临床和预后效用。
遵循系统评价和荟萃分析的首选报告项目（PRISMA）指南。从PubMed、ScienceDirect和谷歌学术数据库中检索相关文献。去除重复文献后，使用纽卡斯尔-渥太华量表评估研究质量。使用I指数评估异质性。如果I大于50%，则使用随机效应模型，否则选择固定效应模型。使用Review Manager（RevMan；版本5.4）进行荟萃分析。
本荟萃分析纳入了5项研究，共494例病例。OSCC中Beclin1的表达与性别、年龄、肿瘤大小、淋巴结转移、组织学分化和总生存期均无显著相关性（p>0.05）。然而，观察到低Beclin1表达有促进肿瘤进展的趋势。敏感性分析显示，低Beclin1表达与显著的淋巴结阳性相关。
本研究概述了OSCC中Beclin1的表达情况，并强调了在将其用作预后标志物时需要进行额外评估。建议未来的研究应评估Beclin1的核表达和胞质表达，并报告其表达在肿瘤内和肿瘤间与临床病理参数相关的差异。
