Su X, Gibor A
Department of Biological Sciences, University of California, Santa Barbara 93106.
Anal Biochem. 1988 Nov 1;174(2):650-7. doi: 10.1016/0003-2697(88)90068-1.
Sulfated, carboxylic polysaccharides and polyphenols found in marine macro-algae interfere with RNA isolation from these plants and inhibit RNA activities in vitro. Methods based on differential precipitation of RNA or carbohydrates in high salts were used to eliminate the acidic carbohydrates. To protect RNA from inactivation by oxidized polyphenols, strong reducing reagents were used to prevent polyphenol oxidation. RNA was successfully isolated from Macro-cystis pyrifera (brown alga), Porphyra schizophylla (red alga), and Enteromorpha intestinalis (green alga). mRNA isolated from the total RNA was shown to be translationally active.
在大型海洋藻类中发现的硫酸化、羧基化多糖和多酚会干扰从这些植物中分离RNA，并在体外抑制RNA活性。基于高盐条件下RNA或碳水化合物差异沉淀的方法被用于去除酸性碳水化合物。为保护RNA不被氧化的多酚失活，使用强还原剂来防止多酚氧化。已成功从巨藻（褐藻）、裂片紫菜（红藻）和肠浒苔（绿藻）中分离出RNA。从总RNA中分离出的mRNA显示具有翻译活性。
