Wang Y, Mu J J, Liu F Q, Ren K Y, Xiao H Y, Yang Z, Yuan Z Y
Cardiovascular Department, First Affiliated Hospital, Medical College, Xi'an Jiaotong University, Xi'an, China.
Department of Pathology, First Affiliated Hospital, Medical College, Xi'an Jiaotong University, Xi'an, China.
Braz J Med Biol Res. 2014 Feb;47(3):223-30. doi: 10.1590/1414-431X20133554. Epub 2014 Mar 18.
Dietary salt intake has been linked to hypertension and cardiovascular disease. Accumulating evidence has indicated that salt-sensitive individuals on high salt intake are more likely to develop renal fibrosis. Epithelial-to-mesenchymal transition (EMT) participates in the development and progression of renal fibrosis in humans and animals. The objective of this study was to investigate the impact of a high-salt diet on EMT in Dahl salt-sensitive (SS) rats. Twenty-four male SS and consomic SS-13(BN) rats were randomized to a normal diet or a high-salt diet. After 4 weeks, systolic blood pressure (SBP) and albuminuria were analyzed, and renal fibrosis was histopathologically evaluated. Tubular EMT was evaluated using immunohistochemistry and real-time PCR with E-cadherin and alpha smooth muscle actin (α-SMA). After 4 weeks, SBP and albuminuria were significantly increased in the SS high-salt group compared with the normal diet group. Dietary salt intake induced renal fibrosis and tubular EMT as identified by reduced expression of E-cadherin and enhanced expression of α-SMA in SS rats. Both blood pressure and renal interstitial fibrosis were negatively correlated with E-cadherin but positively correlated with α-SMA. Salt intake induced tubular EMT and renal injury in SS rats, and this relationship might depend on the increase in blood pressure.
膳食盐摄入量与高血压和心血管疾病有关。越来越多的证据表明，高盐饮食的盐敏感个体更易发生肾纤维化。上皮-间质转化（EMT）参与人和动物肾纤维化的发生和进展。本研究的目的是探讨高盐饮食对 Dahl 盐敏感（SS）大鼠 EMT 的影响。将 24 只雄性 SS 大鼠和同源 SS-13（BN）大鼠随机分为正常饮食组或高盐饮食组。4 周后，分析收缩压（SBP）和蛋白尿，并对肾纤维化进行组织病理学评估。使用免疫组织化学和实时 PCR 检测 E-钙黏蛋白和α平滑肌肌动蛋白（α-SMA）来评估肾小管 EMT。4 周后，与正常饮食组相比，SS 高盐组的 SBP 和蛋白尿显著增加。膳食盐摄入可诱导肾纤维化和肾小管 EMT，表现为 SS 大鼠中 E-钙黏蛋白表达降低和α-SMA 表达增强。血压和肾间质纤维化均与 E-钙黏蛋白呈负相关，但与α-SMA 呈正相关。盐摄入可诱导 SS 大鼠肾小管 EMT 和肾损伤，这种关系可能取决于血压升高。
