金属硫蛋白在雌激素诱导虹鳟（Salmo gairdneri）卵黄蛋白原合成后锌调节中的作用。
A role of metallothionein in zinc regulation after oestradiol induction of vitellogenin synthesis in rainbow trout, Salmo gairdneri.
作者信息
Olsson P E, Zafarullah M, Gedamu L
机构信息
Department of Zoophysiology, University of Göteborg, Sweden.
出版信息
Biochem J. 1989 Jan 15;257(2):555-9. doi: 10.1042/bj2570555.
The regulation of metallothionein (MT) biosynthesis in rainbow-trout liver was studied after a single intraperitoneal injection of oestradiol-17 beta. Sampling was performed after 2, 7, 14, 21, 28 and 35 days. Following induction of vitellogenin synthesis in the liver, liver somatic index (LSI) rose from 1.25 to 2.00 in 14 days. Associated with the increase in LSI was an elevation of hepatic vitellogenin mRNA and zinc concentrations. The vitellogenin mRNA concentrations peaked at 7 days after treatment. The zinc concentrations increased to a peak at day 14. MT was analysed by using differential pulse polarography and a rainbow-trout MT RNA probe. The MT mRNA concentrations rose after 14 days and remained elevated at 21 and 28 days. The MT concentrations increased after 14 days and remained elevated throughout the experimental period. The concentrations of MT-bound zinc increased in association with the elevation in MT concentrations in the oestradiol-treated rainbow trout. These findings indicate that MT is involved in the regulation of zinc during the period of vitellogenin induction and that MT may function by maintaining the pool of available zinc at an appropriate concentration.
在对虹鳟鱼肝脏进行单次腹腔注射17β-雌二醇后，研究了金属硫蛋白（MT）生物合成的调节情况。在注射后2、7、14、21、28和35天进行取样。肝脏中卵黄蛋白原合成被诱导后，肝脏体指数（LSI）在14天内从1.25升至2.00。与LSI升高相关的是肝脏卵黄蛋白原mRNA和锌浓度的升高。卵黄蛋白原mRNA浓度在处理后7天达到峰值。锌浓度在第14天升至峰值。使用差分脉冲极谱法和虹鳟鱼MT RNA探针分析MT。MT mRNA浓度在14天后升高，并在21天和28天保持升高。MT浓度在14天后升高，并在整个实验期间保持升高。在经雌二醇处理的虹鳟鱼中，与MT浓度升高相关的是MT结合锌的浓度增加。这些发现表明，MT在卵黄蛋白原诱导期间参与锌的调节，并且MT可能通过将可用锌库维持在适当浓度来发挥作用。
Zotero 插件