Hashemi Seyed Mohammad Bagher, Shahidi Fakhri, Mortazavi Seyed Ali, Milani Elnaz, Eshaghi Zarrin
Department of Food Science and Technology, College of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran,
Probiotics Antimicrob Proteins. 2014 Mar;6(1):22-31. doi: 10.1007/s12602-014-9155-5.
In this study, the probiotic potential of Lactobacillus strains isolated from traditional Kurdish cheese was investigated. The Lactobacillus strains were examined for resistance to gastric acidity and bile toxicity, antimicrobial activities, autoaggregation, coaggregation, hydrophobicity, adhesion to Caco-2 cells, and antibiotic susceptibility. The results showed that all strains tested tolerate acid gastric conditions (pH 2.0 and 3.0), and all of them were bile resistant (at 0.3 and 1% concentration). Although no antibacterial activity was detected in vitro assay for the treated (neutralized to pH 6.5 and treated with catalase) cell-free culture supernatant (CFCS) of strains, untreated CFCS showed strong antagonistic activity against two known pathogens bacteria. All strains exhibited a strong autoaggregating phenotype and manifested a high degree of coaggregation with pathogens. On the other hand, majority of studied strains were found sensitive to different antibiotics, such as ampicillin, penicillin, ciprofloxacin, chloramphenicol, erythromycin, rifampicin, and tetracycline, and were resistant to vancomycin and streptomycin. Finally, isolated strains showed good hydrophobicity and adherence to Caco-2 cell line, so they could be exploited for food manufacture.
在本研究中，对从传统库尔德奶酪中分离出的乳酸杆菌菌株的益生菌潜力进行了研究。检测了乳酸杆菌菌株对胃酸和胆汁毒性的抗性、抗菌活性、自聚集、共聚集、疏水性、对Caco-2细胞的黏附以及抗生素敏感性。结果表明，所有测试菌株均能耐受胃酸条件（pH 2.0和3.0），并且它们都具有胆汁抗性（浓度为0.3%和1%）。尽管在体外试验中未检测到菌株处理后的（中和至pH 6.5并用过氧化氢酶处理）无细胞培养上清液（CFCS）的抗菌活性，但未处理的CFCS对两种已知病原菌表现出强烈的拮抗活性。所有菌株均表现出强烈的自聚集表型，并与病原菌表现出高度的共聚集。另一方面，大多数研究菌株对不同抗生素敏感，如氨苄青霉素、青霉素、环丙沙星、氯霉素、红霉素、利福平、四环素，对万古霉素和链霉素耐药。最后，分离出的菌株表现出良好的疏水性和对Caco-2细胞系的黏附性，因此可用于食品制造。
