Abbasi Oranus, Mashayekhi Farhad, Mirzajani Ebrahim, Fakhriyeh Asl Saba, Mahmoudi Touraj, Saeedi Saedi Hamid
Department of Cell and Molecular Biology, Pardis International, University of Guilan, Rasht, Iran.
Surg Today. 2015 Feb;45(2):215-20. doi: 10.1007/s00595-014-0886-4. Epub 2014 Mar 28.
VEGFR is involved in complex biological processes, including inflammation and cancer development, progression and metastasis. Many proteins, including VEGFR, are proteolytically released from the surface of cells by a process known as ectodomain shedding. The aim of this study was to assess the expression of soluble VEGFR1 (sVEGFR1) in the serum of patients with colorectal cancer (CRC).
Sixty-two serum samples from healthy controls and 88 samples from patients with different stages of CRC were included in this study. The total protein concentration (TPC) was measured using a Bio-Rad protein assay, and the expression and concentration of sVEGFR1 was determined by a Western blot analysis and enzyme-linked immunosorbent assay, respectively.
No significant difference in the serum TPC of patients with and without CRC was seen. The relative s-VEGFR1 expression and concentration of sVEGFR1 in the serum of patients with CRC were significantly increased compared to those in controls (P < 0.001).
The results of this study suggest that VEGFR1 shedding may provide a reliable and practical indicator of the malignant potential, tumor progression and overall tumor burden. The findings also suggest that sVEGFR1 might be involved in the pathophysiology of CRC, and the detection of serum sVEGFR1 may be useful in classifying CRC.
血管内皮生长因子受体（VEGFR）参与包括炎症和癌症发生、发展及转移在内的复杂生物学过程。许多蛋白质，包括VEGFR，可通过一种称为胞外域脱落的过程从细胞表面被蛋白水解性释放。本研究的目的是评估可溶性VEGFR1（sVEGFR1）在结直肠癌（CRC）患者血清中的表达。
本研究纳入了62份来自健康对照者的血清样本和88份来自不同分期CRC患者的样本。使用Bio-Rad蛋白质测定法测量总蛋白浓度（TPC），并分别通过蛋白质印迹分析和酶联免疫吸附测定法测定sVEGFR1的表达和浓度。
CRC患者和非CRC患者的血清TPC未见显著差异。与对照组相比，CRC患者血清中s-VEGFR1的相对表达和sVEGFR1的浓度显著升高（P < 0.001）。
本研究结果表明，VEGFR1脱落可能为恶性潜能、肿瘤进展和总体肿瘤负荷提供可靠且实用的指标。研究结果还表明，sVEGFR1可能参与CRC的病理生理学过程，血清sVEGFR1的检测可能有助于CRC的分类。
