Hata Tsuyoshi, Takemasa Ichiro, Takahashi Hidekazu, Haraguchi Naotsugu, Nishimura Junichi, Hata Taishi, Mizushima Tsunekazu, Doki Yuichiro, Mori Masaki
Department of Surgery, Gastroenterological Surgery, Graduate School of Medicine, Osaka University, 2-2, Yamadaoka, Suita, Osaka 565-0871, Japan.
Department of Surgery, Surgical Oncology and Science, Sapporo Medical University, S1 W17, Chuo-ku, Sapporo, Hokkaido 060-8556, Japan.
Br J Cancer. 2017 Jul 11;117(2):227-232. doi: 10.1038/bjc.2017.163. Epub 2017 Jun 20.
We previously reported that GTA-446 may be a useful biomarker for early detection of colorectal cancer. In the present study, we confirmed the clinical feasibility of GTA-446 as a screening tool for colorectal cancer with a novel measurement system developed for clinical use. We also improved sensitivity by analysing GTA-446 levels according to gender.
Serum samples were collected from 225 colorectal cancer patients and 916 healthy volunteers to measure GTA-446 levels by flow injection analysis-mass spectrometry.
GTA-446 levels were downregulated in colorectal cancer patients compared with the healthy volunteers, and in females compared with the males in both groups. Receiver operating characteristic curve analysis revealed an optimal cut-off of 2.72 μmol l in males and 1.87 μmol l in females, with a large area under the curve of 0.89-0.93. The sensitivity and specificity were 90.4% and 84.9% for males, 85.2% and 80.5% for females, and 83.3% and 84.8% for all subjects, respectively.
GTA-446 is a clinically relevant biomarker for colorectal cancer with high sensitivity when analysed by gender. Thus, GTA-446 is a promising tool for primary colorectal cancer screening to identify populations at a higher risk of colorectal cancer, with an emphasis on early detection.
我们之前报道过，GTA - 446可能是早期检测结直肠癌的有用生物标志物。在本研究中，我们使用为临床应用开发的新型测量系统，证实了GTA - 446作为结直肠癌筛查工具的临床可行性。我们还通过按性别分析GTA - 446水平提高了检测灵敏度。
收集225例结直肠癌患者和916例健康志愿者的血清样本，采用流动注射分析 - 质谱法测量GTA - 446水平。
与健康志愿者相比，结直肠癌患者的GTA - 446水平下调，且在两组中女性的GTA - 446水平均低于男性。受试者工作特征曲线分析显示，男性的最佳截断值为2.72μmol/L，女性为1.87μmol/L，曲线下面积较大，为0.89 - 0.93。男性的灵敏度和特异性分别为90.4%和84.9%，女性分别为85.2%和80.5%，所有受试者分别为83.3%和84.8%。
GTA - 446是结直肠癌的一种临床相关生物标志物，按性别分析时具有高灵敏度。因此，GTA - 446是一种有前景的原发性结直肠癌筛查工具，可用于识别结直肠癌高风险人群，重点在于早期检测。
