Caruso G, Denaro M G, Caruso R, De Pasquale F, Genovese L, Maricchiolo G
Italian National Research Council, Institute for Coastal Marine Environment, Spianata S. Raineri 86, 98122, Messina, Italy,
Fish Physiol Biochem. 2014 Oct;40(5):1373-82. doi: 10.1007/s10695-014-9931-x. Epub 2014 Mar 28.
An experiment was carried out in red porgy, Pagrus pagrus (Teleostei, Sparidae), to assess the effects of a 14-day fasting period, followed by refeeding to apparent satiation, on the contents of digestive enzymes (total proteases, and particularly pepsin, trypsin, chymotrypsin, carboxypeptidases A and B; amylase and lipase). Two fish groups were considered: one (indicated as fasted/refed group) was fasted for 14 days and then refed during further 7 and 15 days, and the other was fed throughout the study and was taken as a control group. The measured enzymatic values showed that fasting resulted in a generalized, not significant decrease, of the activity of digestive enzymes. Refeeding caused a significant increase for most of the assayed enzymes: total proteases both in the middle and distal intestine, pepsin in the stomach, trypsin in the middle intestine, and amylase and lipase in the proximal intestine. Nevertheless, the detection in the fasted/refed fish of enzymatic values still lower than those measured in the control fish suggested that fish experiencing short-term fasting were partially impaired in their digestive capacity.
在真鲷（Pagrus pagrus，硬骨鱼纲，鲷科）身上进行了一项实验，以评估14天禁食期后再投喂至明显饱足状态对消化酶（总蛋白酶，特别是胃蛋白酶、胰蛋白酶、糜蛋白酶、羧肽酶A和B；淀粉酶和脂肪酶）含量的影响。实验考虑了两组鱼：一组（表示为禁食/再投喂组）禁食14天，然后在接下来的7天和15天进行再投喂，另一组在整个研究过程中持续投喂，作为对照组。测量的酶活性值表明，禁食导致消化酶活性普遍但不显著下降。再投喂使大多数被测酶的活性显著增加：中肠和远端肠中的总蛋白酶、胃中的胃蛋白酶、中肠中的胰蛋白酶以及近端肠中的淀粉酶和脂肪酶。然而，在禁食/再投喂的鱼中检测到的酶活性值仍低于对照组鱼，这表明经历短期禁食的鱼的消化能力部分受损。
