Department of Fisheries, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.
Comp Biochem Physiol A Mol Integr Physiol. 2012 Feb;161(2):166-73. doi: 10.1016/j.cbpa.2011.10.020. Epub 2011 Oct 26.
We evaluated the effects of starvation and refeeding on digestive enzyme activities in juvenile roach, Rutilus rutilus caspicus. Fish were divided into four feeding groups (mean mass 1.68 ± 0.12 g). The control group was fed to satiation twice a day throughout the experiment with formulated diet (SFK). The other three groups were deprived of feed for 1(S1), 2(S2), and 3(S3) weeks, respectively, and then fed to satiation during the refeeding period. The results showed that trypsin specific activity was not affected significantly either by starvation or refeeding, in all experimental groups. Chymotrypsin specific activity did not change significantly in S1 fish during the experimental period. In S2 and S3 fish no significant changes were observed during the starvation period. Upon refeeding, the activity increased in S2 fish, while it decreased in S3 fish. Amylase specific activity decreased significantly during the starvation period in all experimental groups. Upon refeeding, the activity increased. Alkaline phosphatase specific activity did not change significantly during the experiment period in S3 fish, while it showed significant changes during the starvation and refeeding period in the S1 and S2 fish. Starvation also had a significant effect on the structure of the intestine.
我们评估了饥饿和再投喂对幼体鲤鱼（Rutilus rutilus caspicus）消化酶活性的影响。将鱼分为四个投喂组（平均质量为 1.68 ± 0.12 g）。对照组在整个实验期间每天用配方饲料（SFK）投喂两次以达到饱食状态。另外三组分别饥饿 1（S1）、2（S2）和 3（S3）周，然后在再投喂期间喂至饱食。结果表明，在所有实验组中，无论是饥饿还是再投喂，胰蛋白酶比活力均不受显著影响。在实验期间，S1 组的糜蛋白酶比活力没有显著变化。在 S2 和 S3 组的饥饿期间，没有观察到明显的变化。再投喂时，S2 组的活性增加，而 S3 组的活性降低。在所有实验组中，淀粉酶比活力在饥饿期间显著降低。再投喂时，活性增加。碱性磷酸酶比活力在 S3 组的实验期间没有显著变化，而在 S1 和 S2 组的饥饿和再投喂期间表现出显著变化。饥饿对肠道结构也有显著影响。
