Tian Ye, Teng Li-rong, Song Jing-jing, Meng Qing-fan, Lu Jia-hui, Zhang Wei-wei, Wei Kang, Wang Ning, Wang Di, Teng Le-sheng
College of Life Sciences, Jilin University, No. 2699, Qianjin Avenue, Changchun City, Jilin, 130012, People's Republic of China.
Protoplasma. 2014 Sep;251(5):1245-53. doi: 10.1007/s00709-014-0637-9. Epub 2014 Mar 28.
The analgesic activity of Porcellio laevis Latreille, Rhizoma Corydalis, and Radix Cynanchi Paniculati have been reported in recent years. A new formula named Jia-Yuan-Qing pill (JYQP) is therefore created by combining the three herbs at 9:7:7 ratio according to traditional Chinese theories. The present study aims to evaluate the effect of JYQP as a novel painkiller in various models. Acute toxicity test was applied to evaluate the safety of JYQP. Acetic-acid-induced writhing, hot plate test, formalin test, and naloxone-pretreated writhing test were employed to elaborate the analgesic activity of JYQP and its possible mechanism. A bone cancer pain mouse model was performed to further assess the effect of JYQP in relieving cancer pain. Test on naloxone-precipitated withdrawal symptoms was conduct to examine the physical dependence of mice on JYQP. Data revealed that JYQP reduced writhing and stretching induced by acetic acid; however, this effect could not be blocked by naloxone. JYQP specifically suppressed the phase II reaction time in formalin-treated mice; meanwhile, no analgesic effect of JYQP in hot plate test was observed, indicating that JYQP exerts analgesic activity against inflammatory pain rather than neurogenic pain. Furthermore, JYQP could successfully relieve bone cancer pain in mice. No physical dependence could be observed upon long-term administration in mice. Collectively, our present results provide experimental evidence in supporting clinical use of JYQP as an effective and safe agent for pain treatment.
近年来，人们已报道了光滑鼠妇、延胡索和徐长卿的镇痛活性。因此，根据传统中医理论，将这三种草药按9:7:7的比例组合，创制了一种名为加元清丸（JYQP）的新配方。本研究旨在评估JYQP作为一种新型止痛药在各种模型中的效果。应用急性毒性试验来评估JYQP的安全性。采用醋酸诱导扭体试验、热板试验、福尔马林试验和纳洛酮预处理扭体试验来阐述JYQP的镇痛活性及其可能的作用机制。构建骨癌疼痛小鼠模型以进一步评估JYQP缓解癌痛的效果。进行纳洛酮诱发戒断症状试验以检测小鼠对JYQP的身体依赖性。数据显示，JYQP可减少醋酸诱导的扭体和伸展反应；然而，这种作用不能被纳洛酮阻断。JYQP可特异性抑制福尔马林处理小鼠的Ⅱ期反应时间；同时，在热板试验中未观察到JYQP的镇痛作用，这表明JYQP对炎性疼痛而非神经源性疼痛具有镇痛活性。此外，JYQP可成功缓解小鼠的骨癌疼痛。长期给小鼠给药未观察到身体依赖性。总体而言，我们目前的结果为支持JYQP作为一种有效且安全的疼痛治疗药物的临床应用提供了实验证据。
