Roch J M, Tosic M, Roach A, Matthieu J M
Laboratoire de Neurochimie, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland.
Brain Res. 1989 Jan 16;477(1-2):292-9. doi: 10.1016/0006-8993(89)91417-0.
Myelin basic protein (MBP) gene organization and expression were analyzed in wild type and myelin deficient (mld) mutant mice. Southern analysis demonstrated MBP gene duplication in mld mice. In addition, we present evidence that one MBP gene in mld mice is normal for at least 14 kilobases (kb) upstream from exon I, whereas the second gene is normal for at least 3.5 kb but not more than 7 kb upstream from exon I. Run-on experiments showed that the rate of MBP gene transcription in mld mice is similar to that seen in normal mice. Detailed analysis of the transcriptional activity of various regions of the gene led us to conclude that all portions of the MBP gene are transcribed in mld mice. Consequently, we propose that the low levels of MBP mRNA observed in these mice (2-5% of the wild-type level) are not due to deficient transcriptional activity.
在野生型和髓鞘缺乏（mld）突变小鼠中分析了髓鞘碱性蛋白（MBP）基因的组织和表达。Southern分析表明mld小鼠中存在MBP基因重复。此外，我们提供的证据表明，mld小鼠中的一个MBP基因在 exon I 上游至少14千碱基（kb）是正常的，而第二个基因在 exon I 上游至少3.5 kb但不超过7 kb是正常的。连续转录实验表明，mld小鼠中MBP基因的转录速率与正常小鼠相似。对该基因各个区域转录活性的详细分析使我们得出结论，MBP基因的所有部分在mld小鼠中都有转录。因此，我们认为在这些小鼠中观察到的低水平MBP mRNA（野生型水平的2-5%）并非由于转录活性不足。
