Bertl Kristina, Zijnge Vincent, Zatorska Beata, Leonhard Matthias, Schneider-Stickler Berit, Harmsen Hermie J M
Division of Oral Surgery, Bernhard Gottlieb School of Dentistry, Medical University of Vienna, Vienna, Austria.
Head Neck. 2015 Apr;37(4):524-9. doi: 10.1002/hed.23625. Epub 2014 Apr 9.
A polymerase chain reaction (PCR)-based method has been used to identify oral anaerobic pathogens in biofilms on voice prostheses. The purpose of the present study was to determine the location of those pathogens inside the biofilms.
Biofilms of 15 voice prostheses were sampled and used to identify the oral pathogens. Fluorescence in situ hybridization was applied on smears made on glass slides and on sections of intact biofilms visualized by confocal laser scanning microscopy (CLSM).
Fusobacterium nucleatum (F. nucleatum) was the most frequently detected pathogen and the only tested species detected in microcolonies. The other microbes (Parvimonas micra [P. micra], Porphyromonas gingivalis [P. gingivalis], Tannerella forsythia [T. forsythia], and Treponema denticola [T. denticola]) were not detected or only detected as single cells. CLSM analysis showed that F. nucleatum resided on the biofilm surface.
Although detectable, oral anaerobic pathogens seem to be no more than passers-by that adhere without further observed proliferation and apparently play no striking role in biofilm formation on voice prostheses.
一种基于聚合酶链反应（PCR）的方法已被用于鉴定语音假体生物膜中的口腔厌氧病原体。本研究的目的是确定这些病原体在生物膜内的位置。
对15个语音假体的生物膜进行采样，并用于鉴定口腔病原体。荧光原位杂交应用于载玻片上制备的涂片以及通过共聚焦激光扫描显微镜（CLSM）可视化的完整生物膜切片。
具核梭杆菌（F. nucleatum）是最常检测到的病原体，也是在微菌落中检测到的唯一测试物种。其他微生物（微小单胞菌[P. micra]、牙龈卟啉单胞菌[P. gingivalis]、福赛坦氏菌[T. forsythia]和具齿密螺旋体[T. denticola]）未被检测到或仅作为单个细胞被检测到。CLSM分析表明具核梭杆菌位于生物膜表面。
尽管可检测到，但口腔厌氧病原体似乎只是过客，它们粘附在语音假体上，但未观察到进一步增殖，显然在语音假体生物膜形成中没有显著作用。
