Karahaliloğlu Zeynep, Ercan Batur, Chung Stanley, Taylor Erik, Denkbaş Emir B, Webster Thomas J
Nanotechnology and Nanomedicine Division, Hacettepe University, 06800, Beytepe, Ankara, Turkey.
J Biomed Mater Res A. 2014 Dec;102(12):4598-608. doi: 10.1002/jbm.a.35141. Epub 2014 Mar 5.
Major issues faced with the use of today's skin grafts are infection, scar tissue formation, insufficient keratinocyte (or skin producing cells) proliferation and high production costs. To overcome these limitations, we propose here for the first time, a nanofeatured poly(lactide-co-glycolide) (PLGA) membrane as a next generation antibacterial skin graft material. An alkaline surface treatment method was used to create random nanofeatures on PLGA membranes where sodium hydroxide (NaOH) concentration and exposure times were altered to control surface morphology. Most significantly, and without the use of antibiotics, results showed a decrease in Staphylococcus aureus (a dangerous pathogen infecting skin grafts) growth for up to ∼40% after 2 days of culture on nanofeatured PLGA membranes compared to untreated controls. Results also showed that while bacteria growth was stunted, mammalian cell growth was not. Specifically, cell culture results showed an increase in human epidermal keratinocyte density, while the density of scar tissue forming human dermal fibroblasts, did not change on nanofeatured PLGA surfaces compared to the untreated controls after 3 days of culture. These findings indicate that the alkaline treatment of PLGA membranes is a promising quick and effective manner to limit scar tissue formation and bacterial invasion while increasing skin cell proliferation for improving numerous wound-healing applications.
当今皮肤移植面临的主要问题包括感染、瘢痕组织形成、角质形成细胞（或皮肤生成细胞）增殖不足以及生产成本高昂。为克服这些限制，我们首次在此提出一种具有纳米特征的聚（丙交酯 - 乙交酯）（PLGA）膜作为下一代抗菌皮肤移植材料。采用碱性表面处理方法在PLGA膜上制造随机纳米特征，通过改变氢氧化钠（NaOH）浓度和暴露时间来控制表面形态。最为显著的是，在不使用抗生素的情况下，结果显示与未处理的对照相比，在具有纳米特征的PLGA膜上培养2天后，金黄色葡萄球菌（一种感染皮肤移植的危险病原体）的生长减少了约40%。结果还表明，虽然细菌生长受到抑制，但哺乳动物细胞生长不受影响。具体而言，细胞培养结果显示人表皮角质形成细胞密度增加，而在具有纳米特征的PLGA表面上，培养3天后，形成瘢痕组织的人真皮成纤维细胞密度与未处理的对照相比没有变化。这些发现表明，PLGA膜的碱性处理是一种有前景的快速有效的方法，可限制瘢痕组织形成和细菌入侵，同时增加皮肤细胞增殖，以改善众多伤口愈合应用。
