Lin Yinan, Brown Roger F, Jung Steven B, Day Delbert E
Department of Biological Sciences, Missouri University of Science and Technology, Rolla, Missouri, 65409.
J Biomed Mater Res A. 2014 Dec;102(12):4491-9. doi: 10.1002/jbm.a.35120. Epub 2014 Feb 26.
The primary objective of this research was to evaluate the use of bioactive borate-based glass microfibers for angiogenesis in soft tissue repair applications. The effect of these fibers on growth of capillaries and small blood vessels was compared to that of 45S5 silica glass microfibers and sham implant controls. Compressed mats of three types of glass microfibers were implanted subcutaneously in rats and tissues surrounding the implant sites histologically evaluated 2-4 weeks post surgery. Bioactive borate glass 13-93B3 supplemented with 0.4 wt % copper promoted extensive angiogenesis as compared to silica glass microfibers and sham control tissues. The angiogenic responses suggest the copper-containing 13-93B3 microfibers may be effective for treating chronic soft tissue wounds. A second objective was to assess the possible systemic cytotoxicity of dissolved borate ions and other materials released from implanted borate glass microfibers. Cytotoxicity was assessed via histological evaluation of kidney tissue collected from animals 4 weeks after subcutaneously implanting high amounts of the borate glass microfibers. The evaluation of the kidney tissue from these animals showed no evidence of chronic histopathological changes in the kidney. The overall results indicate the borate glass microfibers are safe and effective for soft tissue applications.
本研究的主要目的是评估生物活性硼酸盐基玻璃微纤维在软组织修复应用中促进血管生成的作用。将这些纤维对毛细血管和小血管生长的影响与45S5二氧化硅玻璃微纤维及假植入对照组进行比较。将三种类型的玻璃微纤维压缩垫皮下植入大鼠体内，并在术后2 - 4周对植入部位周围的组织进行组织学评估。与二氧化硅玻璃微纤维和假对照组织相比，添加了0.4 wt%铜的生物活性硼酸盐玻璃13 - 93B3促进了广泛的血管生成。血管生成反应表明含铜的13 - 93B3微纤维可能对治疗慢性软组织伤口有效。第二个目的是评估植入的硼酸盐玻璃微纤维释放的溶解硼离子和其他物质可能产生的全身细胞毒性。通过对皮下植入大量硼酸盐玻璃微纤维4周后动物采集的肾脏组织进行组织学评估来评估细胞毒性。对这些动物肾脏组织的评估显示，肾脏没有慢性组织病理学变化的迹象。总体结果表明，硼酸盐玻璃微纤维在软组织应用中是安全有效的。
