Guo Baojian, Chen Yanhong, Li Chuan, Wang Tianya, Wang Rui, Wang Bo, Hu Sha, Du Xiaofen, Xing Hongyan, Song Xiao, Yao Yingyin, Sun Qixin, Ni Zhongfu
State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization (MOE), Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing, China; National Plant Gene Research Centre (Beijing), Beijing, China.
Proteomics. 2014 May;14(9):1071-87. doi: 10.1002/pmic.201300147.
To better understand the underlying molecular basis of leaf development in maize, a reference map of nuclear proteins in basal region of seedling leaf was established using a combination of 2DE and MALDI-TOF-MS. In total, 441 reproducible protein spots in nuclear proteome of maize leaf basal region were detected with silver staining in a pH range of 3-10, among which 203 spots corresponding to 163 different proteins were identified. As expected, proteins implicated in RNA and protein-associated functions were overrepresented in nuclear proteome. Remarkably, a high percentage (10%) of proteins was identified to be involved in cell division and growth. In addition, comparative nuclear proteomic analysis in leaf basal region of highly heterotic hybrid Mo17/B73 and its parental lines was also performed and 52 of 445 (11.69%) detected protein spots were differentially expressed between the hybrid and its parental lines, among which 16 protein spots displayed nonadditively expressed pattern. These results indicated that hybridization between two parental lines can cause changes in the expression of a variety of nuclear proteins, which may be responsible for the observed leaf size heterosis.
为了更好地理解玉米叶片发育的潜在分子基础，结合二维电泳（2DE）和基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS）建立了玉米幼苗叶片基部区域核蛋白的参考图谱。通过银染法在pH值3-10范围内总共检测到玉米叶片基部区域核蛋白质组中的441个可重复蛋白质点，其中鉴定出对应于163种不同蛋白质的203个点。正如预期的那样，与RNA和蛋白质相关功能有关的蛋白质在核蛋白质组中占比过高。值得注意的是，有高比例（10%）的蛋白质被鉴定为参与细胞分裂和生长。此外，还对高度杂种优势的杂交种Mo17/B73及其亲本系的叶片基部区域进行了比较核蛋白质组分析，在杂交种与其亲本系之间检测到的445个蛋白质点中有52个（11.69%）差异表达，其中16个蛋白质点呈现非加性表达模式。这些结果表明两个亲本系之间的杂交可导致多种核蛋白表达的变化，这可能是观察到的叶片大小杂种优势的原因。
