van Binnendijk R S, Poelen M C, de Vries P, Voorma H O, Osterhaus A D, Uytdehaag F G
Department of Immunobiology, National Institute of Public Health and Environmental Protection, Bilthoven, The Netherlands.
J Immunol. 1989 Apr 15;142(8):2847-54.
PBMC from healthy adult individuals seropositive for measles virus (MV) were tested for their capacity to proliferate to UV-inactivated MV (UV-MV) or to autologous MV-infected EBV-transformed B cell lines (EBV-BC). MV-specific T cell responses were observed in 11 of 15 donors tested (stimulation index greater than 2), when optimal doses of UV-MV were used in proliferative assays. T cell clones were generated from PBMC of three donors responding to MV, by using either UV-MV or MV-infected autologous EBV-BC as APC. Stimulation with UV-MV generated exclusively CD3+ CD4+ CD8- MV-specific T cells, whereas after stimulation of PBMC with MV-infected EBV-BC, both CD3+ CD4+ CD8- and CD3+ CD4- CD8+ MV-specific T cell clones were obtained. Of 19 CD4+ T cell clones tested so far, 7 clones reacted specifically with purified fusion protein and 1 with purified hemagglutinin protein. Seven clones proliferated in response to the internal proteins of MV. Three clones reacted to whole virus but not to one of the purified proteins, whereas one clone seemed to recognize more than one polypeptide. Some of the T cell clones, generated from in vitro stimulation of PBMC with UV-MV, failed to recognize MV Ag when MV-infected EBV-BC were used as APC instead of UV-MV and PBMC. CD3+ CD4+ CD8- T cell clones recognized MV in association with HLA class II Ag (HLA-DQ or -DR), and most of them displayed CTL activity to autologous MV-infected EBV-BC. All CD4+ HLA class II-restricted CTL clones thus far tested were capable of assisting B lymphocytes for the production of MV-specific antibody. The CD4- CD8+ T cell clone MARO 1 recognized MV in association with HLA class I molecules and displayed cytotoxic activity toward MV-infected EBV-BC.
对麻疹病毒（MV）血清反应呈阳性的健康成年个体的外周血单核细胞（PBMC）进行检测，以评估其对紫外线灭活的MV（UV-MV）或自体MV感染的EBV转化B细胞系（EBV-BC）的增殖能力。在增殖试验中使用最佳剂量的UV-MV时，在15名受试供体中的11名中观察到了MV特异性T细胞反应（刺激指数大于2）。通过使用UV-MV或MV感染的自体EBV-BC作为抗原呈递细胞（APC），从三名对MV有反应的供体的PBMC中产生T细胞克隆。用UV-MV刺激仅产生CD3 + CD4 + CD8 - MV特异性T细胞，而在用MV感染的EBV-BC刺激PBMC后，获得了CD3 + CD4 + CD8 - 和CD3 + CD4 - CD8 + MV特异性T细胞克隆。在迄今为止测试的19个CD4 + T细胞克隆中，7个克隆与纯化的融合蛋白特异性反应，1个与纯化的血凝素蛋白反应。7个克隆对MV的内部蛋白有增殖反应。3个克隆对完整病毒有反应，但对其中一种纯化蛋白无反应，而1个克隆似乎能识别不止一种多肽。一些通过用UV-MV体外刺激PBMC产生的T细胞克隆，当用MV感染的EBV-BC代替UV-MV和PBMC作为APC时，无法识别MV抗原。CD3 + CD4 + CD8 - T细胞克隆与HLA II类抗原（HLA-DQ或-DR）结合识别MV，并且它们中的大多数对自体MV感染的EBV-BC表现出细胞毒性活性。迄今为止测试的所有CD4 + HLA II类限制性CTL克隆都能够辅助B淋巴细胞产生MV特异性抗体。CD4 - CD8 + T细胞克隆MARO 1与HLA I类分子结合识别MV，并对MV感染的EBV-BC表现出细胞毒性活性。
