Jacobson S, Richert J R, Biddison W E, Satinsky A, Hartzman R J, McFarland H F
J Immunol. 1984 Aug;133(2):754-7.
We have generated measles virus-specific T cell clones from a patient with multiple sclerosis who has been described previously as a strong responder to measles virus. T cell clones were screened on the basis of their capacity to proliferate to measles virus. The cell surface phenotype of each clone was OKT3+, OKT4+, OKT8-. The majority of these clones (11 of 14) were cytotoxic for their autologous measles virus-infected lymphoblastoid B cell target. This cytotoxicity was specific for measles virus inasmuch as these T cell clones could not lyse influenza or mumps virus-infected B cell targets. By using a panel of HLA-defined measles virus-infected B cell lines, these clones were shown to recognize measles virus in the context of HLA class II determinants. A monoclonal antibody that recognizes HLA class II monomorphic determinants (L243), but not a monoclonal antibody that recognizes HLA class I antigens (W6/32), inhibited the lysis of the autologous measles virus-infected B cell target by these T cell clones. These results demonstrate that these cytotoxic T cell clones specific for measles virus are HLA class II restricted.
我们从一名多发性硬化症患者身上获得了麻疹病毒特异性T细胞克隆，该患者之前被描述为对麻疹病毒有强烈反应。根据T细胞克隆对麻疹病毒的增殖能力进行筛选。每个克隆的细胞表面表型为OKT3 +、OKT4 +、OKT8 -。这些克隆中的大多数（14个中的11个）对其自身感染麻疹病毒的淋巴母细胞样B细胞靶标具有细胞毒性。这种细胞毒性对麻疹病毒具有特异性，因为这些T细胞克隆不能裂解感染流感或腮腺炎病毒的B细胞靶标。通过使用一组HLA定义的感染麻疹病毒的B细胞系，这些克隆显示在HLA II类决定簇的背景下识别麻疹病毒。一种识别HLA II类单态决定簇的单克隆抗体（L243），而不是识别HLA I类抗原的单克隆抗体（W6/32），抑制了这些T细胞克隆对自身感染麻疹病毒的B细胞靶标的裂解。这些结果表明，这些针对麻疹病毒的细胞毒性T细胞克隆受HLA II类限制。
