对在突尼斯检测到的人类A组轮状病毒株的NSP4蛋白进行的序列和结构分析。
Sequence and structural analyses of NSP4 proteins from human group A rotavirus strains detected in Tunisia.
作者信息
Ben Hadj Fredj M, Ben Hamida-Rebaï M, Zeller M, Heylen E, Van Ranst M, Matthijnssens J, Trabelsi A
机构信息
UR06SP20, Laboratory of Microbiology, Sahloul University Hospital, 4054 Sousse, Tunisia; Faculty of Pharmacy, University of Monastir, avenue Avicenne, 5019 Monastir, Tunisia.
Laboratory of Clinical and Epidemiological Virology, Department of Microbiology and Immunology, Rega Institute for Medical Research, University of Leuven, 1, place de l'Université, 1348 Louvain-La-Neuve, Belgium.
出版信息
Pathol Biol (Paris). 2014 Jun;62(3):146-51. doi: 10.1016/j.patbio.2013.10.006. Epub 2014 Mar 26.
BACKGROUND
The NSP4 protein of group A rotavirus (RVA) has been recognized as a viral enterotoxin and plays important roles in viral pathogenesis and morphogenesis. Domains involved in structural and functional interactions have been proposed mainly based on the simian SA11 strain.
METHODS
NSP4 has been classified into 15 different genotypes (E1-E15), and the aim of this study was to analyze the sequences of 46 RVA strains in order to determine the aminoacid (aa) differences between E1 and E2 genotypes. Another aspect was to characterize the structural and physicochemical properties of these strains.
RESULTS
Comparison of deduced aa sequences of the NSP4 protein showed that divergences between NSP4 genotypes E1 and E2 were mostly observed in the VP4-binding, the interspecies variable domain (ISVD) and the double-layered particle (DLP) binding domains. Interestingly, uncommon variations in residues 131 and 138, which are known to be important aa in pathogenesis, were found in one unusual animal derived strain belonging to the E2 genotype. Concerning the structural aspect, no significant differences were noted.
CONCLUSION
The presence of punctual aa variations in the NSP4 genotypes may indicate that NSP4 mutates mainly via accumulation of point mutations.
背景
A组轮状病毒（RVA）的NSP4蛋白已被确认为一种病毒肠毒素，在病毒发病机制和形态发生中发挥重要作用。参与结构和功能相互作用的结构域主要是基于猿猴SA11株提出的。
方法
NSP4已被分为15种不同的基因型（E1-E15），本研究的目的是分析46株RVA毒株的序列，以确定E1和E2基因型之间的氨基酸（aa）差异。另一个方面是表征这些毒株的结构和物理化学性质特性。
结果
NSP4蛋白推导的氨基酸序列比较表明，NSP4基因型E1和E2之间的差异主要出现在VP4结合区、种间可变区（ISVD）和双层颗粒（DLP）结合区。有趣的是，在一株属于E2基因型异常动物源毒株中，发现了已知在发病机制中重要的第131和138位残基的罕见变异。从结构方面来看，未发现显著差异。
结论
NSP4基因型中存在点状氨基酸变异可能表明NSP4主要通过点突变的积累发生突变。
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