Department of Analytical Chemistry, Physical Chemistry and Chemical Engineering, Faculty of Biology, Environmental Sciences and Chemistry, University of Alcalá, Ctra. Madrid-Barcelona Km. 33.600, 28871 Alcalá de Henares, Madrid, Spain.
Department of Analytical Chemistry, Physical Chemistry and Chemical Engineering, Faculty of Biology, Environmental Sciences and Chemistry, University of Alcalá, Ctra. Madrid-Barcelona Km. 33.600, 28871 Alcalá de Henares, Madrid, Spain.
Food Chem. 2014 Aug 15;157:62-9. doi: 10.1016/j.foodchem.2014.01.130. Epub 2014 Feb 12.
This work evaluates, the presence of native antihypertensive peptides in five soybean-based infant formulas (SBIFs). SBIFs peptide extracts (<10 kDa) and their sub-fractions (5-10 kDa, 3-5 kDa, and <3 kDa) from a variety of samples were obtained by ultrafiltration and ACE inhibitory activity was determined. The highest activities were observed in the smaller (<5 kDa) peptide fractions. A set of peptides present in various SBIFs were studied, and identified using HPLC-Q-ToF-MS. Despite ACE inhibitory activity decreasing after in vitro gastrointestinal digestion, it still remained at a high value (IC50 values of 18.2±0.1 and 4.9±0.1 μg/mL). Peptides resisting the action of gastrointestinal enzymes were identified and compared to previously identified peptides, highlighting the presence of peptide RPSYT. This peptide was synthesised, its antihypertensive and antioxidant activity were evaluated, and its resistance to in vitro gastrointestinal digestion and to high processing temperatures were studied.
本研究评估了五种大豆配方婴儿食品（SBIF）中天然降压肽的存在。通过超滤获得了 SBIF 肽提取物（<10 kDa）及其亚馏分（5-10 kDa、3-5 kDa 和 <3 kDa），并测定了它们的 ACE 抑制活性。在较小的 (<5 kDa) 肽馏分中观察到最高的活性。研究了存在于各种 SBIF 中的一组肽，并使用 HPLC-Q-ToF-MS 进行了鉴定。尽管体外胃肠道消化后 ACE 抑制活性降低，但仍保持在较高水平（IC50 值分别为 18.2±0.1 和 4.9±0.1 μg/mL）。鉴定了抵抗胃肠道酶作用的肽，并与先前鉴定的肽进行了比较，突出了肽 RPSYT 的存在。该肽被合成，评估了其降压和抗氧化活性，并研究了其对体外胃肠道消化和高温加工的抗性。
