Gaviria G Yhoan S, Guerra Carlos M, Zapata M José E
Nutrition and Food Technology Research Group, Universidad de Antioquia, calle 70 No. 52-21, Medellín, Colombia.
Grupo de investigación GIRYSOUT, Universidad del Tolima, Ibagué, Colombia.
Heliyon. 2024 Feb 2;10(3):e25715. doi: 10.1016/j.heliyon.2024.e25715. eCollection 2024 Feb 15.
Hypertension is an important risk factor concomitant with cardiovascular disease (CVD) states, which is why we set out to evaluate Californian red worm hydrolysates on antihypertensive activity both , , using rabbit aortic rings and using hypertensive induced rats. The worms were manually separated, washed with water, purged for 4 h with 4 % sodium bicarbonate, sacrificed with 7 % saline, and finally washed with drinking water. The antihypertensive capacity was performed by measuring angiotensin-converting enzyme inhibition; for the assays, rabbit aorta was used to measure relaxation; for the assays, rats with induced hypertension were used to perform acute (hypotension) and chronic assays, using captopril as a control in all assays. With respect to angiotensin-converting enzyme (ACE) inhibition, the EC50 value of the worm hydrolysate was found to be 358 ppm; with respect to the analysis in aortic rings, it was found that the mechanisms of action of the hydrolysate are endothelium-dependent, presenting a maximum relaxation of 35 %. With respect to the assays, the hypotensive test showed that the hydrolysate can reduce blood pressure by up to 32 % in only 2 h, while the chronic analysis showed that the hydrolysate at 150 ppm did not present statistically significant differences with the control (captopril) during the 15 days of analysis. The Red Californian earthworm hydrolysate presents bioactive compounds identified with antihypertensive activities , and in different isolated and animal models. The study demonstrates the efficacy of the hydrolysate to be used as an alternative in the treatment and prevention of hypertension, and it can be implemented in functional foods or nutraceutical foods. Antihypertensive peptides, particularly those that inhibit angiotensin-converting enzyme (ACE), hold significant importance in medical research, specifically in the context of cardiovascular disease treatment, particularly hypertension. The focus on these peptides and the potential implications of their results in medical research can be summarized through several key points: 1) Mechanisms of Action-Antihypertensive peptides function by inhibiting ACE or renin, crucial enzymes in blood pressure regulation. 2)Alternatives to Synthetic Drugs, 3) Additional Health Benefits, and various other factors.
高血压是与心血管疾病（CVD）状态相关的重要风险因素，这就是为什么我们着手评估加利福尼亚红虫水解产物对降压活性的影响，既使用兔主动脉环进行体外评估，又使用高血压诱导大鼠进行体内评估。将 worms 手动分离，用水冲洗，用4%碳酸氢钠净化4小时，用7%盐水处死，最后用饮用水冲洗。通过测量血管紧张素转换酶抑制来测定降压能力；对于体外实验，使用兔主动脉来测量舒张；对于体内实验，使用高血压诱导大鼠进行急性（低血压）和慢性实验，在所有实验中均使用卡托普利作为对照。关于血管紧张素转换酶（ACE）抑制，发现虫水解产物的EC50值为358 ppm；关于主动脉环分析，发现水解产物的作用机制是内皮依赖性的，最大舒张率为35%。关于体内实验，降压测试表明，水解产物在仅2小时内可使血压降低多达32%，而慢性分析表明，在15天的分析期间，150 ppm的水解产物与对照（卡托普利）相比没有统计学上的显著差异。加利福尼亚红蚯蚓水解产物呈现出具有降压活性的生物活性化合物，在不同的分离物和动物模型中均有体现。该研究证明了水解产物作为高血压治疗和预防替代品的有效性，并且可以应用于功能性食品或营养食品中。降压肽，特别是那些抑制血管紧张素转换酶（ACE）的降压肽，在医学研究中具有重要意义，特别是在心血管疾病治疗，尤其是高血压的背景下。对这些肽及其结果在医学研究中的潜在影响的关注可以通过几个关键点来概括：1）作用机制 - 降压肽通过抑制ACE或肾素发挥作用，ACE和肾素是血压调节中的关键酶。2）合成药物的替代品，3）额外的健康益处，以及各种其他因素。 （注：原文中worms未明确是什么，这里保留英文未翻译）
