Miroshnichenko V P, Savel'ev V G
Lab Delo. 1989(1):18-20.
Studies of bilirubin absorption spectra by protein measurements with the use of the biuret test and Lowry's method have shown that bilirubin influences much the protein absorption spectrum, provoking higher results in examinations of the bile. To eliminate bilirubin effects, the authors recommend bilirubin extraction with ethyl-acetone mixture in a 1:1 ratio after protein sedimentation with trichloroacetic acid. The biuret test with bilirubin-free protein yields results compatible with those obtained by nonphotometric techniques not involving bilirubin effects.
通过使用双缩脲试验和洛瑞法进行蛋白质测量对胆红素吸收光谱的研究表明，胆红素对蛋白质吸收光谱有很大影响，在胆汁检测中会导致更高的结果。为消除胆红素的影响，作者建议在用三氯乙酸沉淀蛋白质后，用1:1比例的乙酸乙酯混合物提取胆红素。对不含胆红素的蛋白质进行双缩脲试验，其结果与通过不涉及胆红素影响的非光度技术获得的结果相符。
