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[考马斯亮蓝G 250法测定蛋白质。IV. 用于脑脊液蛋白质及与双缩脲法和洛瑞法的比较分析]

[Determination of proteins with the Coomassie brilliant blue G 250 method. IV. Use with cerebrospinal fluid proteins and comparative analysis with the biuret and Lowry methods].

作者信息

Serra S, Morgante L, Di Perri R

出版信息

Boll Soc Ital Biol Sper. 1980 Mar 15;56(5):463-7.

PMID:7387789
Abstract

The method of Bradford with Coomassie Brillant Blue G 250 for protein assay has been applyed to CSF in comparison with biuret and Lowry's methods. The results of Coomassie method closely agree with Lowry, and are similar to those of biuret. It is suggested to replace the previously used methods for CSF protein assay by the Bradford's method, because it keeps the sensitivity of Lowry and the simplicity of biuret. It adds too the advantage of requiring less time.

摘要

采用考马斯亮蓝G 250的Bradford法进行蛋白质测定,并与双缩脲法和Lowry法一起应用于脑脊液检测。考马斯法的结果与Lowry法非常吻合,且与双缩脲法的结果相似。建议用Bradford法取代先前用于脑脊液蛋白质检测的方法,因为它兼具Lowry法的灵敏度和双缩脲法的简便性。它还具有所需时间较少的优点。

相似文献

1
[Determination of proteins with the Coomassie brilliant blue G 250 method. IV. Use with cerebrospinal fluid proteins and comparative analysis with the biuret and Lowry methods].[考马斯亮蓝G 250法测定蛋白质。IV. 用于脑脊液蛋白质及与双缩脲法和洛瑞法的比较分析]
Boll Soc Ital Biol Sper. 1980 Mar 15;56(5):463-7.
2
[Determination of proteins with the Coomassie brilliant blue G 250 method. III. Behavior of various pure proteins and comparative analysis with the biuret and Lowry methods].[考马斯亮蓝G 250法测定蛋白质。III. 各种纯蛋白质的行为及与双缩脲法和洛里法的比较分析]
Boll Soc Ital Biol Sper. 1980 Mar 15;56(5):458-62.
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[Method of determination of proteins with Coomassie brilliant blue G 250. I. General characteristics and comparative analysis with the biuret method and Lowry's method].[考马斯亮蓝G 250法测定蛋白质。I. 一般特性及与双缩脲法和洛瑞法的比较分析]
Boll Soc Ital Biol Sper. 1980 Jan 30;56(2):160-5.
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Cerebrospinal fluid protein assay with Coomassie Brilliant Blue G 250: a comparative analysis with different methods.考马斯亮蓝G 250脑脊液蛋白测定法:与不同方法的比较分析
Acta Neurol (Napoli). 1980 Apr;2(2):94-101.
5
[Bradford's method of determining protein: application, advantages and disadvantages].[布拉德福德蛋白质测定法:应用、优点与缺点]
Lab Delo. 1989(4):4-7.
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[Quantitative determination of total urinary protein utilizing the principle of Coomassie Brilliant Blue G250 binding to protein (author's transl)].利用考马斯亮蓝G250与蛋白质结合原理对尿总蛋白进行定量测定(作者译)
J Clin Chem Clin Biochem. 1981 Apr;19(4):203-8.
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The quantitation and fractionation of proteins in cerebrospinal fluid.脑脊液中蛋白质的定量与分级分离
Am J Med Technol. 1982 Oct;48(10):821-7.
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[Determination of protein in cerebrospinal fluid: comparison of the Coomassie-Blue method and the Cu-Folin differential test].[脑脊液中蛋白质的测定:考马斯亮蓝法与铜-福林比色法的比较]
J Clin Chem Clin Biochem. 1983 Mar;21(3):151-5.
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[Use of dye binding for quantitative determination of proteins in solutions (review)].[利用染料结合法对溶液中的蛋白质进行定量测定(综述)]
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Protein measurement of particulate and solubilized ovine liver membranes.颗粒状和可溶解的绵羊肝细胞膜的蛋白质测定
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