Suppr超能文献

[考马斯亮蓝G 250法测定蛋白质。IV. 用于脑脊液蛋白质及与双缩脲法和洛瑞法的比较分析]

[Determination of proteins with the Coomassie brilliant blue G 250 method. IV. Use with cerebrospinal fluid proteins and comparative analysis with the biuret and Lowry methods].

作者信息

Serra S, Morgante L, Di Perri R

出版信息

Boll Soc Ital Biol Sper. 1980 Mar 15;56(5):463-7.

PMID:7387789
Abstract

The method of Bradford with Coomassie Brillant Blue G 250 for protein assay has been applyed to CSF in comparison with biuret and Lowry's methods. The results of Coomassie method closely agree with Lowry, and are similar to those of biuret. It is suggested to replace the previously used methods for CSF protein assay by the Bradford's method, because it keeps the sensitivity of Lowry and the simplicity of biuret. It adds too the advantage of requiring less time.

摘要

采用考马斯亮蓝G 250的Bradford法进行蛋白质测定,并与双缩脲法和Lowry法一起应用于脑脊液检测。考马斯法的结果与Lowry法非常吻合,且与双缩脲法的结果相似。建议用Bradford法取代先前用于脑脊液蛋白质检测的方法,因为它兼具Lowry法的灵敏度和双缩脲法的简便性。它还具有所需时间较少的优点。

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验