Kuznetsova G G
Lab Delo. 1989(1):51-3.
The author has developed a micromodification of the rapid photoemulsion method for the detection of the microorganism proteolytic activity. The test is carried out on polystyrene plates, meat-peptone broth is used as the suspension fluid. Rectangular film strips are vertically placed into the wells, thus preventing false-negative reactions possible in case of an erroneous horizontal position of the film with the emulsion layer turned upwards if square or round film fragments are used. The proteolytic activities of 120 microorganism strains (95 of these hydrolyze gelatin) have been examined by the micromethod and the routine test with gelatin. The suggested test is 2-4-fold more rapid than the routine one used in investigations of the cultures slowly hydrolyzing gelatin. The fact that the test is carried out on the plates considerably reduces the nutrient medium and the number of laboratory glassware and helps obtain more accurate results.
作者研发了一种用于检测微生物蛋白水解活性的快速光乳化法的微改进方法。该测试在聚苯乙烯板上进行，肉蛋白胨肉汤用作悬浮液。将矩形薄膜条垂直放入孔中，这样如果使用方形或圆形薄膜碎片，可防止在薄膜乳液层向上翻转时水平放置错误而可能出现的假阴性反应。已通过这种微方法和常规明胶测试检查了120株微生物菌株（其中95株能水解明胶）的蛋白水解活性。对于在研究中缓慢水解明胶的培养物，建议的测试比常规测试快2至4倍。该测试在平板上进行这一事实大大减少了营养培养基的用量和实验室玻璃器皿的数量，并有助于获得更准确的结果。
