Yamasaki Kazumitsu, Yoshida Kaoru, Yoshiike Miki, Shimada Kazuhiko, Nishiyama Hiroyuki, Takamizawa Satoru, Yanagida Kaoru, Iwamoto Teruaki
Department of Urology, Tsukuba Gakuen Hospital, Tsukuba, Japan.
Center for IVF and Infertility, International University of Health and Welfare Hospital, Nasushiobara, Japan.
Basic Clin Androl. 2017 Aug 8;27:15. doi: 10.1186/s12610-017-0059-6. eCollection 2017.
Semenogelins (SEMGs) are major components of human seminal vesicle secretions. Due to SEMG's sperm-motility inhibitor, a significant negative correlation between sperm motility and the proportion of SEMG-bound spermatozoa (SEMG+) was found in asthenozoospermic patients. SEMGs also show intrinsic inhibitory capability for sperm capacitation; however, studies on actual clinical specimens have not been conducted.
To reveal the relationship between SEMGs and the fertilizing capacity of sperm from male infertile patients who are not restricted to asthenozoospermia, we measured the proportion of SEMG+ in the spermatozoa of 142 male infertile patients. The pregnancy outcomes in partners of these patients were retrospectively analyzed using questionnaires.
Among examined semen parameters, only the total SEMG-unbound sperm count showed a tendency to be different between the spontaneous pregnancy or intra-uterine-insemination-pregnancy groups and in-vitro-fertilization- or intracytoplasmic-sperm-injection-pregnancy groups. It was elevated in the former group, which includes patients who used in vivo fertilization.
The total SEMG-unbound sperm count would be a relevant parameter for in vivo fertilization. This result suggests that SEMGs inhibit ectopic capacitation before sperm reach the fertilization site and that the number of total SEMG-unbound sperm is a parameter directly linked to the possibility of in vivo fertilization.
精液凝胶蛋白（SEMGs）是人类精囊分泌物的主要成分。由于SEMG具有抑制精子活力的作用，在弱精子症患者中发现精子活力与结合SEMG的精子比例（SEMG+）之间存在显著负相关。SEMGs对精子获能也具有内在抑制能力；然而，尚未对实际临床标本进行研究。
为了揭示SEMGs与不限于弱精子症的男性不育患者精子受精能力之间的关系，我们测量了142例男性不育患者精子中SEMG+的比例。使用问卷对这些患者配偶的妊娠结局进行回顾性分析。
在所检测的精液参数中，只有未结合SEMG的精子总数在自然妊娠或宫腔内人工授精妊娠组与体外受精或卵胞浆内单精子注射妊娠组之间显示出差异趋势。在前一组（包括采用体内受精的患者）中该数值升高。
未结合SEMG的精子总数可能是体内受精的一个相关参数。这一结果表明，SEMGs在精子到达受精部位之前抑制异位获能，并且未结合SEMG的精子总数是与体内受精可能性直接相关的一个参数。
