利多卡因对体外培养的马空肠平滑肌耐去污剂膜中浮舰蛋白-2分布的影响。

Lidocaine effect on flotillin-2 distribution in detergent-resistant membranes of equine jejunal smooth muscle in vitro.

作者信息

Tappenbeck Karen, Schmidt Sonja, Feige Karsten, Naim Hassan Y, Huber Korinna

机构信息

Department of Physiology, University of Veterinary Medicine, Bischofsholer Damm 15, 30173 Hannover, Germany.

Department of Physiological Chemistry, University of Veterinary Medicine, Bünteweg 17, 30559 Hannover, Germany.

出版信息

Vet J. 2014 May;200(2):325-7. doi: 10.1016/j.tvjl.2014.03.002. Epub 2014 Mar 26.

DOI:10.1016/j.tvjl.2014.03.002

PMID:24680669

Abstract

Lidocaine is the most commonly chosen prokinetic for treating postoperative ileus in horses, a motility disorder associated with ischaemia-reperfusion injury of intestinal tissues. Despite the frequent use of lidocaine, the mechanism underlying its prokinetic effects is still unclear. Previous studies suggested that lidocaine altered cell membrane characteristics of smooth muscle cells. Therefore, the present study aimed to elucidate effects of lidocaine administration on characteristics of detergent-resistant membranes in equine jejunal smooth muscle. Lidocaine administration caused significant redistribution of flotillin-2, a protein marker of detergent-resistant membranes, in fractions of sucrose-density-gradients obtained from ischaemia-reperfusion injured smooth muscle solubilised with Triton X-100. It was concluded that lidocaine induced disruption of detergent-resistant membranes which might affect ion channel activity and therefore enhance smooth muscle contractility.

摘要

利多卡因是治疗马术后肠梗阻最常用的促动力药，肠梗阻是一种与肠组织缺血再灌注损伤相关的动力障碍。尽管利多卡因使用频繁，但其促动力作用的潜在机制仍不清楚。先前的研究表明，利多卡因改变了平滑肌细胞的细胞膜特性。因此，本研究旨在阐明利多卡因给药对马空肠平滑肌耐去污剂膜特性的影响。利多卡因给药导致从用Triton X-100溶解的缺血再灌注损伤平滑肌获得的蔗糖密度梯度组分中，耐去污剂膜的蛋白质标志物flotillin-2发生显著重新分布。得出的结论是，利多卡因诱导耐去污剂膜的破坏，这可能影响离子通道活性，从而增强平滑肌收缩力。