Kaslow D C, Syin C, McCutchan T F, Miller L H
Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892.
Mol Biochem Parasitol. 1989 Mar 15;33(3):283-7. doi: 10.1016/0166-6851(89)90090-x.
The gene encoding the 25 kDa ookinete surface antigen (Pgs25) of Plasmodium gallinaceum has been cloned using an oligonucleotide probe directed against one of the EGF-like domains of the P. falciparum 25 kDa ookinete surface antigen (Pfs25). The Pgs25 gene codes for a polypeptide of 215 amino acids, two amino residues less than Pfs25. The deduced amino acid sequence contains a putative signal sequence at the amino-terminus, four tandemly repeated EGF-like domains, and a hydrophobic region at the carboxyl-terminus. By comparing Pgs25 with Pfs25, six conserved regions, consisting of six or more amino acid residues, have been identified. Most of the conserved regions are outside EGF-like core consensus sequences. The most striking conservation is the spacing of the cysteines.
已使用针对恶性疟原虫25 kDa动合子表面抗原（Pfs25）的一个表皮生长因子（EGF）样结构域的寡核苷酸探针，克隆了鸡疟原虫25 kDa动合子表面抗原（Pgs25）的编码基因。Pgs25基因编码一个由215个氨基酸组成的多肽，比Pfs25少两个氨基酸残基。推导的氨基酸序列在氨基末端含有一个假定的信号序列、四个串联重复的EGF样结构域以及在羧基末端的一个疏水区。通过将Pgs25与Pfs25进行比较，已鉴定出六个由六个或更多氨基酸残基组成的保守区域。大多数保守区域位于EGF样核心共有序列之外。最显著的保守之处是半胱氨酸的间距。
