Ji Peng, Wei Yanming, Xue Wenxin, Hua Yongli, Zhang Man, Sun Hongguo, Song Zhixue, Zhang Ling, Li Jinxia, Zhao Haifu, Zhang Wenquan
Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, PR China.
Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, PR China.
Int J Biol Macromol. 2014 Jun;67:195-200. doi: 10.1016/j.ijbiomac.2014.03.025. Epub 2014 Mar 26.
Five polysaccharides from unprocessed Chinese angelica (UCAP), parched one with alcohol (ACAP), soil (SCAP), sesame oil (OCAP) and parched into charred (CCAP) were extracted and purified. Their structures were identified by Fourier transform-infrared spectroscopy (FT-IR), compositions were analyzed by gas chromatography-mass spectrometry (GC-MS) and antioxidative activities were compared by determining MDA contents and SOD activities of liver tissue in mice damaged with CCl4 after gavage. The results showed that the FT-IR spectra of CCAP and OCAP displayed lower transmittance at around 1050cm(-1) in comparison with that of UCAP. Five polysaccharides were all composed of rhamnose, arabinose, mannose, glucose and galactose. In CCAP, ACAP, OCAP and SCAP, the proportions of arabinose were significantly increased in comparison with that of UCAP. The SOD activities in CCAP and SCAP groups were significantly enhanced, and MDA contents in CCAP, OCAP and SCAP groups were significantly decreased as compared with UCAP group. This indicated that processing could change the structure, composition and enhance antioxidative activity of polysaccharide in Chinese angelica, and CCAP possessed the strongest antioxidative activity.
从生当归（UCAP）、酒炙当归（ACAP）、土炒当归（SCAP）、油炒当归（OCAP）和炒炭当归（CCAP）中提取并纯化了5种多糖。通过傅里叶变换红外光谱（FT-IR）鉴定其结构，采用气相色谱-质谱联用（GC-MS）分析其组成，并通过测定四氯化碳损伤小鼠灌胃后肝脏组织的丙二醛（MDA）含量和超氧化物歧化酶（SOD）活性来比较其抗氧化活性。结果表明，与UCAP相比，CCAP和OCAP的FT-IR光谱在1050cm(-1)左右处的透光率较低。5种多糖均由鼠李糖、阿拉伯糖、甘露糖、葡萄糖和半乳糖组成。与UCAP相比，CCAP、ACAP、OCAP和SCAP中阿拉伯糖的比例显著增加。与UCAP组相比，CCAP组和SCAP组的SOD活性显著增强，CCAP组、OCAP组和SCAP组的MDA含量显著降低。这表明炮制可改变当归多糖的结构、组成并增强其抗氧化活性，且CCAP具有最强的抗氧化活性。
