Sengoda Venkatesan G, Cooper W Rodney, Swisher Kylie D, Henne Donald C, Munyaneza Joseph E
Yakima Agricultural Research Laboratory, Agricultural Research Service, United States Department of Agriculture, Wapato, Washington, United States of America; Department of Entomology, Subtropical Pest Management Laboratory, Texas A&M AgriLife Research, Weslaco, Texas, United States of America.
Yakima Agricultural Research Laboratory, Agricultural Research Service, United States Department of Agriculture, Wapato, Washington, United States of America.
PLoS One. 2014 Mar 28;9(3):e93475. doi: 10.1371/journal.pone.0093475. eCollection 2014.
"Candidatus Liberibacter solanacearum" (Lso) is an economically important pathogen of solanaceous crops and the putative causal agent of zebra chip disease of potato (Solanum tuberosum L.). This pathogen is transmitted to solanaceous species by the potato psyllid, Bactericera cockerelli (Šulc), but many aspects of the acquisition and transmission processes have yet to be elucidated. The present study was conducted to assess the interacting effects of acquisition access period, incubation period, and host plant on Lso titer in psyllids, the movement of Lso from the alimentary canal to the salivary glands of the insect, and the ability of psyllids to transmit Lso to non-infected host plants. Following initial pathogen acquisition, the probability of Lso presence in the alimentary canal remained constant from 0 to 3 weeks, but the probability of Lso being present in the salivary glands increased with increasing incubation period. Lso copy numbers in psyllids peaked two weeks after the initial pathogen acquisition and psyllids were capable of transmitting Lso to non-infected host plants only after a two-week incubation period. Psyllid infectivity was associated with colonization of insect salivary glands by Lso and with Lso copy numbers >10,000 per psyllid. Results of our study indicate that Lso requires a two-week latent period in potato psyllids and suggest that acquisition and transmission of Lso by psyllids follows a pattern consistent with a propagative, circulative, and persistent mode of transmission.
“番茄致病变种韧皮杆菌”（Lso）是茄科作物的一种具有经济重要性的病原体，也是马铃薯（Solanum tuberosum L.）斑马薯片病的假定致病因子。这种病原体通过马铃薯木虱Bactericera cockerelli（Šulc）传播到茄科植物，但获取和传播过程的许多方面仍有待阐明。本研究旨在评估获取接入期、潜伏期和寄主植物对木虱体内Lso滴度、Lso从昆虫消化道向唾液腺的移动以及木虱将Lso传播到未感染寄主植物的能力的相互作用影响。在最初获取病原体后，Lso存在于消化道中的概率在0至3周内保持恒定，但Lso存在于唾液腺中的概率随着潜伏期的增加而增加。木虱体内的Lso拷贝数在最初获取病原体两周后达到峰值，并且木虱只有在两周的潜伏期后才能将Lso传播到未感染的寄主植物。木虱的感染性与Lso在昆虫唾液腺中的定殖以及每只木虱的Lso拷贝数>10,000有关。我们的研究结果表明，Lso在马铃薯木虱中需要两周的潜伏期，并表明木虱获取和传播Lso遵循与增殖性、循环性和持久性传播模式一致的模式。
