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在美国发现番茄潜叶蛾（半翅目：潜蛾科）的第四种单倍型

Identification of a fourth haplotype of Bactericera cockerelli (Hemiptera: Triozidae) in the United States.

作者信息

Swisher Kylie D, Henne Donald C, Crosslin James M

机构信息

United States Department of Agriculture, Agricultural Research Service, Prosser, WA, 99350

Texas A&M AgriLife Research, Subtropical Pest Management Laboratory, Weslaco, TX, 78596.

出版信息

J Insect Sci. 2014 Jan 1;14:161. doi: 10.1093/jisesa/ieu023. Print 2014.

DOI:10.1093/jisesa/ieu023
PMID:25368079
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5443600/
Abstract

The potato psyllid, Bactericera cockerelli (Sulc) (Hemiptera: Triozidae), is a pest of potato and other solanaceous crops in North and Central America and New Zealand. Previous genotyping studies have demonstrated the presence of three different haplotypes of B. cockerelli in the United States corresponding to three geographical regions: Central, Western, and Northwestern. These studies utilized psyllids collected in the western and central United States between 1998 and 2011. In an effort to further genotype potato psyllids collected in the 2012 growing season, a fourth B. cockerelli haplotype was discovered corresponding to the Southwestern United States geographical region. High-resolution melting analyses identified this new haplotype using an amplicon generated from a portion of the B. cockerelli mitochondrial cytochrome c oxidase subunit I gene. Sequencing of this gene, as well as use of a restriction enzyme assay, confirmed the identification of the novel B. cockerelli haplotype in the United States.

摘要

马铃薯木虱，即茄无网长管蚜（Sulc）（半翅目：木虱科），是北美洲、中美洲和新西兰马铃薯及其他茄科作物的一种害虫。先前的基因分型研究表明，在美国存在与三个地理区域相对应的三种不同的茄无网长管蚜单倍型，即中部、西部和西北部。这些研究利用了1998年至2011年期间在美国西部和中部采集的木虱。为了进一步对2012年生长季节采集的马铃薯木虱进行基因分型，发现了与美国西南部地理区域相对应的第四种茄无网长管蚜单倍型。高分辨率熔解分析使用从茄无网长管蚜线粒体细胞色素c氧化酶亚基I基因的一部分生成的扩增子鉴定了这种新的单倍型。对该基因进行测序以及使用限制性酶切分析，证实了在美国鉴定出新型茄无网长管蚜单倍型。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9131/5443600/b4a34c207787/ieu023f5p.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9131/5443600/404cf74a146f/ieu023f1p.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9131/5443600/c98e171ea728/ieu023f2p.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9131/5443600/61b44db32af0/ieu023f3p.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9131/5443600/a4ea6c9042d9/ieu023f4p.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9131/5443600/b4a34c207787/ieu023f5p.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9131/5443600/404cf74a146f/ieu023f1p.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9131/5443600/c98e171ea728/ieu023f2p.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9131/5443600/61b44db32af0/ieu023f3p.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9131/5443600/a4ea6c9042d9/ieu023f4p.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9131/5443600/b4a34c207787/ieu023f5p.jpg

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