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鸡虱(Eomenacanthus stramineus,昆虫纲,食毛目)卵母细胞中副核的形成与功能。I. 超微结构和组织化学研究

Formation and function of accessory nuclei in the oocytes of the bird louse, Eomenacanthus stramineus (Insecta, Mallophaga). I. Ultrastructural and histochemical studies.

作者信息

Biliński S M

机构信息

Institute of Zoology, Jagiellonian University, Kraków, Poland.

出版信息

Chromosoma. 1989 Jan;97(4):321-6. doi: 10.1007/BF00371973.

DOI:10.1007/BF00371973
PMID:2468458
Abstract

In the oocytes of Eomenacanthus stramineus accessory nuclei arise by budding from the nuclear envelope. It is suggested that microtubules and the thick layer of the nuclear lamina are involved in this process. Newly formed accessory nuclei contain aggregations of fibrillogranular material. These aggregations are slightly Feulgen positive, RNA negative and stain positively with the Ag-NOR method. During later developmental stages one dense, RNA-positive inclusion appears in each accessory nucleus. These inclusions consist of an Ag-NOR-positive cortical layer and an Ag-NOR-negative core. The function of accessory nuclei in the species investigated is discussed in the light of these results.

摘要

在淡黄棘腹蛛的卵母细胞中,副核由核膜出芽形成。有人认为微管和核纤层的厚层参与了这一过程。新形成的副核含有纤维颗粒物质的聚集体。这些聚集体对福尔根反应呈弱阳性,对RNA呈阴性,用银染核仁组织区(Ag-NOR)方法呈阳性染色。在发育后期,每个副核中出现一个致密的、RNA阳性的内含物。这些内含物由一个银染核仁组织区阳性的皮质层和一个银染核仁组织区阴性的核心组成。根据这些结果,讨论了所研究物种中副核的功能。

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引用本文的文献

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Subcortical microtubule network separates the periplasm from the endoplasm and is responsible for maintaining the position of accessory nuclei in hymenopteran oocytes.皮层下微管网络将周质与内质分离,并负责维持膜翅目昆虫卵母细胞中副核的位置。
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A new version of the Ag-NOR technique. A combination with DAPI staining.

本文引用的文献

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OVARIAN DEVELOPMENT IN HABROBRACON JUGLANDIS (ASHMEAD) (HYMENOPTERA: BRACONIDAE). I. THE ORIGIN AND DIFFERENTIATION OF THE OOCYTENURSE CELL COMPLEX.
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Ultrastructural localization of Ag-NOR stained proteins in the nucleolus during the cell cycle and in other nucleolar structures.细胞周期中核仁及其他核仁结构内银染核仁组织区(Ag-NOR)染色蛋白的超微结构定位
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