Boloukhère M
J Cell Sci. 1984 Jan;65:73-93. doi: 10.1242/jcs.65.1.73.
Silver staining at the electron microscopic level of the nucleolar organizers was carried out on Xenopus laevis oocytes at various stages of oogenesis. The results indicate that a positive reaction takes place exclusively in the dense fibrillar component of the extrachromosomal nucleoli. This constituent undergoes morphological changes of distribution and architecture, which have been correlated with modifications of the transcriptional activity of the nucleoli. When nucleolar activity is reduced, during previtellogenesis, this constituent appears as dense homogeneous spherules well-segregated from the granular component. In contrast, when nucleolar activity is high, during vitellogenesis, it forms an heterogeneous area with an ill-delimited outline: it is organized into a fibrillar core with emerging skein-like strings. It thus seems that this constituent remains silver-stained throughout oogenesis. These findings suggest that the method used would allow one to follow the evolution of the nucleolar organizer region (NOR) topography during oogenesis. Moreover, they point out facts that have relevance to the problem of the correlation between Ag stainability of NORs and nucleolar transcriptional activity.
在电子显微镜水平上，对处于不同卵母细胞发生阶段的非洲爪蟾卵母细胞的核仁组织者进行了银染。结果表明，阳性反应仅发生在染色体外核仁的致密纤维成分中。该成分经历了分布和结构的形态变化，这些变化与核仁转录活性的改变相关。在前卵黄发生期核仁活性降低时，该成分表现为与颗粒成分明显分离的致密均匀球体。相反，在卵黄发生期核仁活性较高时，它形成一个轮廓不清晰的异质区域：它被组织成一个带有出现的丝状串的纤维核心。因此，在整个卵母细胞发生过程中，该成分似乎一直保持银染。这些发现表明，所使用的方法将使人们能够追踪卵母细胞发生过程中核仁组织者区域（NOR）拓扑结构的演变。此外，它们指出了与NORs的银染色性和核仁转录活性之间相关性问题相关的事实。
