Varga G, Papp M, Dobronyi I, Scarpignato C
Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest.
Digestion. 1988;41(4):229-36. doi: 10.1159/000199795.
The effect of equimolar doses (6 nmol/kg) of bombesin and its mammalian counterpart, GRP, on pancreatic growth and secretion was studied in adult rats. Both peptides were administered intraperitoneally three times a day for 5 consecutive days. Saline-treated rats were used as controls. At the end of the treatment, animals were anaesthetized and pancreatic juice was collected in basal conditions and after caerulein (0.75 nmol/kg i.p.) stimulation. Afterwards, the rats were sacrificed and growth and composition of the pancreatic tissue were determined. Compared with the control (saline) values, either basal or stimulated secretion was significantly increased after short-term treatment with both peptides. In addition, both bombesin and GRP increased pancreatic weight, total pancreatic protein, trypsin and amylase content. The DNA content was also increased by both peptides, although only the GRP effect proved to be significant. These results demonstrate that both bombesin and GRP have a growth-promoting effect on rat pancreas and concomitantly increase its secretory capacity. The mechanism of this peculiar biological action is likely to be connected with a direct stimulatory action on the gland.
在成年大鼠中研究了等摩尔剂量（6纳摩尔/千克）的蛙皮素及其哺乳动物对应物胃泌素释放肽（GRP）对胰腺生长和分泌的影响。两种肽均每天腹腔注射3次，连续注射5天。用生理盐水处理的大鼠作为对照。在治疗结束时，将动物麻醉，并在基础状态下以及注射蛙皮素（0.75纳摩尔/千克，腹腔注射）刺激后收集胰液。之后，处死大鼠并测定胰腺组织的生长和组成。与对照（生理盐水）值相比，用两种肽进行短期治疗后，基础分泌或刺激分泌均显著增加。此外，蛙皮素和GRP均增加了胰腺重量、胰腺总蛋白、胰蛋白酶和淀粉酶含量。两种肽也使DNA含量增加，尽管只有GRP的作用被证明具有显著性。这些结果表明，蛙皮素和GRP均对大鼠胰腺具有促生长作用，并同时增加其分泌能力。这种特殊生物学作用的机制可能与对腺体的直接刺激作用有关。
