Damgé C, Hajri A, Lhoste E, Aprahamian M
INSERM Unit 61, Digestive Cell Biology and Physiopathology, Strasbourg, France.
Regul Pept. 1988 Feb;20(2):141-50. doi: 10.1016/0167-0115(88)90047-x.
This study was designed to compare, on a molar basis, the effect of chronic bombesin, gastrin-releasing peptide (GRP) and caerulein on pancreatic growth in the rat. These 3 peptides were administered s.c. 3 times daily for 4 days at the following concentrations: 0.036, 0.36, 3.6 and 7.2 nmol/kg of body weight. Bombesin and GRP induced pancreatic growth in a dose-dependent manner from 3.6 nmol/kg. This growth was characterized by an increase in pancreatic weight, its protein and RNA contents but not in DNA content suggesting cellular hypertrophy. Caerulein exerted a biphasic effect on pancreatic growth, inducing cellular hypertrophy at low doses since 0.36 nmol/kg and atrophy with the highest dose (7.2 nmol/kg). Bombesin and caerulein (until 3.6 nmol/kg) increased the pancreatic content in chymotrypsin more than in amylase. The 7.2 nmol/kg caerulein treatment depressed all enzyme activities while the same dose of GRP increased pancreatic lipase content. It is concluded that (1) bombesin and GRP are equipotent trophic factors for the pancreas; (2) caerulein is the most potent factor and exerts a biphasic effect on pancreatic growth; (3) pancreatic growth and synthesis and/or secretion of enzymes are not regulated through the same mechanism.
本研究旨在以摩尔为基础，比较慢性给予蛙皮素、胃泌素释放肽（GRP）和雨蛙肽对大鼠胰腺生长的影响。这三种肽以以下浓度每日皮下注射3次，共4天：0.036、0.36、3.6和7.2 nmol/kg体重。从3.6 nmol/kg起，蛙皮素和GRP以剂量依赖性方式诱导胰腺生长。这种生长的特征是胰腺重量、蛋白质和RNA含量增加，但DNA含量未增加，提示细胞肥大。雨蛙肽对胰腺生长产生双相作用，自0.36 nmol/kg起低剂量时诱导细胞肥大，而高剂量（7.2 nmol/kg）时导致萎缩。蛙皮素和雨蛙肽（直至3.6 nmol/kg）使胰凝乳蛋白酶的胰腺含量增加幅度大于淀粉酶。7.2 nmol/kg雨蛙肽处理降低了所有酶活性，而相同剂量的GRP增加了胰腺脂肪酶含量。结论为：（1）蛙皮素和GRP是对胰腺同等有效的营养因子；（2）雨蛙肽是最有效的因子，对胰腺生长产生双相作用；（3）胰腺生长以及酶的合成和/或分泌并非通过相同机制调节。
