Preibisch G, Ishihara H, Tripier D, Leineweber M
Department of Biochemistry, Hoechst AG, Frankfurt, F.R.G.
Gene. 1988 Dec 10;72(1-2):179-86. doi: 10.1016/0378-1119(88)90141-2.
When expressing several eukaryotic genes in Escherichia coli, we observed N-terminally truncated proteins which were attributed to translation initiation at downstream AUG codons. These AUG codons are located between 4 and 20 nucleotides 3' from sequences resembling bacterial SD elements. Although the presence of such downstream SD sequences is not sufficient for downstream initiation to occur, in two cases their removal abolishes synthesis of the truncated proteins. In one construct, a potential hairpin-loop structure is likely to inhibit translation initiation at the correct site and favor downstream initiation.
当在大肠杆菌中表达几个真核基因时，我们观察到了N端截短的蛋白质，这归因于在下游AUG密码子处的翻译起始。这些AUG密码子位于距类似于细菌SD元件的序列3'端4至20个核苷酸之间。尽管这种下游SD序列的存在不足以使下游起始发生，但在两种情况下，去除它们会消除截短蛋白的合成。在一个构建体中，一个潜在的发夹环结构可能会抑制正确位点的翻译起始，并有利于下游起始。
