Inamine J M, Loechel S, Hu P C
Department of Pediatrics, University of North Carolina, Chapel Hill 27599-7220.
Gene. 1988 Dec 15;73(1):175-83. doi: 10.1016/0378-1119(88)90323-x.
The attachment of virulent Mycoplasma pneumoniae to the ciliated epithelium of the respiratory tract involves a surface protein designated P1. Our previous determination of the nucleotide sequence of the P1 attachment-protein gene revealed that it is flanked by open reading frames (ORFs) and there is no obvious ribosome-binding site (RBS) or transcription termination sequence in the adjacent regions. We extended this analysis by cloning and sequencing the 18-kb region containing the P1 gene. This study indicates that the P1 gene is transcribed as part of a larger polycistronic message. The P1 operon is composed of the P1 gene and two predicted genes, designated ORF-4 and ORF-6. The gene order is ORF-4, P1, ORF-6 with intervening regions of 12 and 5 nt, respectively. ORF-4 and ORF-6 have respective coding capacities for proteins of Mr approximately equal to 28,000 and Mr approximately equal to 130,000. Putative promoter and RBS sequences which correspond closely to those found in Escherichia coli and Bacillus subtilis, as well as a sequence indicative of a transcription terminator, have been found in the flanking sequences. The transcription start point has been determined by primer extension of M. pneumoniae RNA.
毒力肺炎支原体附着于呼吸道的纤毛上皮涉及一种名为P1的表面蛋白。我们之前对P1附着蛋白基因核苷酸序列的测定表明，它两侧是开放阅读框（ORF），相邻区域没有明显的核糖体结合位点（RBS）或转录终止序列。我们通过克隆和测序包含P1基因的18 kb区域扩展了这一分析。这项研究表明，P1基因作为一个更大的多顺反子信息的一部分被转录。P1操纵子由P1基因和两个预测基因组成，分别命名为ORF-4和ORF-6。基因顺序为ORF-4、P1、ORF-6，中间区域分别为12和5个核苷酸。ORF-4和ORF-6分别对分子量约为28,000和分子量约为130,000的蛋白质具有编码能力。在侧翼序列中发现了与大肠杆菌和枯草芽孢杆菌中发现的序列紧密对应的推定启动子和RBS序列，以及一个指示转录终止子的序列。转录起始点已通过肺炎支原体RNA的引物延伸确定。
