Key Laboratory of Automobile Materials of MOE and State Key Laboratory of Superhard Materials, Department of Materials Science, Jilin University, Changchun 130012, People׳s Republic of China.
Key Laboratory of Automobile Materials of MOE and State Key Laboratory of Superhard Materials, Department of Materials Science, Jilin University, Changchun 130012, People׳s Republic of China.
Biosens Bioelectron. 2014 Aug 15;58:374-9. doi: 10.1016/j.bios.2014.03.002. Epub 2014 Mar 12.
The binding of DNA with graphene oxide (GO) is important for applications in disease diagnosis, genetic screening, and drug discovery. The standard assay methods are mainly limited to indirect observation via fluorescence labeling. Here we report the use of surface plasmon resonance for direct sensing of DNA/GO binding. We show that this can be used for ultra-sensitive detection of single-stranded DNA (ssDNA). Furthermore, the results provide a more direct probe of DNA/GO binding abilities and confirm that hydrogen bonding plays a key role in the interaction between GO and ssDNA. This enables to a novel biosensor for highly sensitive and selective detection of ssDNA based on indirect competitive inhibition assay (ICIA). We report development of such a sensor with a linear dynamic range of 10(-14)-10(-6)M, a detection limit of 10fM and a high level of stability during repeated regeneration.
氧化石墨烯（GO）与 DNA 的结合对于疾病诊断、基因筛查和药物发现等应用非常重要。标准的检测方法主要局限于通过荧光标记进行间接观察。在这里，我们报告了使用表面等离子体共振（SPR）进行 DNA/GO 结合的直接传感。我们表明，这可用于超灵敏检测单链 DNA（ssDNA）。此外，这些结果提供了对 DNA/GO 结合能力的更直接探针，并证实氢键在 GO 和 ssDNA 之间的相互作用中起着关键作用。这使得基于间接竞争抑制分析（ICIA）的 ssDNA 高度敏感和选择性检测的新型生物传感器成为可能。我们报告了这种传感器的开发，其线性动态范围为 10(-14)-10(-6)M，检测限为 10fM，并且在重复再生过程中具有高水平的稳定性。
