Ying Hao, Liu Jinpeng, Du Qizhen
Institute of Food Science, Zhejiang A & F University, Linan 311300, Hangzhou, China.
Institute of Food Science, Zhejiang A & F University, Linan 311300, Hangzhou, China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2014 May 1;958:36-42. doi: 10.1016/j.jchromb.2014.03.006. Epub 2014 Mar 15.
Amomum longiligulare or Amomum villosum showed oestrogenic activity. In the present study, oestrogen-active components in fructus amomi, the seeds of A. longiligulare were separated by high-speed countercurrent chromatography (HSCCC) using stepwise elution of eight mobile phases with gradient polarity and advanced separation by high performance liquid chromatography (HPLC). The results yielded 17 compounds with the amount of 8-138mg and a purity of 94.3-99.8% from a 3g ethanolic extract of fructus amomi. The chemical structures of the compounds were identified by ESI-MS and NMR spectra, in which eight diarylheptanoids were demonstrated as the main oestrogen-active compounds in the fructus amomi. Determination of the diarylheptanoids in fructus amomi from various origins showed that fructus amomi contains more than 0.5% total diarylheptanoids. The results showed that fructus amomi is a diarylheptanoids-rich food resource possessing oestrogen-activity. The combination method of HSCCC and HPLC can be applied for the analysis of bioactive compounds by detecting the corresponding bioactivity in the HSCCC fractions and separating the target compounds with HPLC.
长柄豆蔻或砂仁显示出雌激素活性。在本研究中，利用高速逆流色谱法（HSCCC）通过逐步洗脱八种具有梯度极性的流动相，并结合高效液相色谱法（HPLC）进行进一步分离，从长柄豆蔻种子（即砂仁）中分离出具有雌激素活性的成分。结果从3g砂仁乙醇提取物中得到了17种化合物，含量为8 - 138mg，纯度为94.3 - 99.8%。通过电喷雾电离质谱（ESI-MS）和核磁共振谱（NMR）对这些化合物的化学结构进行了鉴定，其中八种二芳基庚烷类化合物被证明是砂仁中主要的雌激素活性化合物。对不同产地砂仁中二芳基庚烷类化合物的测定表明，砂仁中总二芳基庚烷类化合物含量超过0.5%。结果表明，砂仁是一种富含二芳基庚烷类化合物且具有雌激素活性的食物资源。HSCCC和HPLC的联用方法可通过检测HSCCC馏分中的相应生物活性以及利用HPLC分离目标化合物来应用于生物活性化合物的分析。
