Sun Ting-Ting, Liang Xin-Lei, Zhu He-Yun, Peng Xu-Ling, Guo Xing-Jie, Zhao Long-Shan
School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, PR China.
J Ginseng Res. 2016 Jul;40(3):220-8. doi: 10.1016/j.jgr.2015.07.008. Epub 2015 Aug 5.
Among the various ginseng strains, Shizhu ginseng is endemic to China, mainly distributed in Kuandian Manchu Autonomous County (Liaoning Province, China); however, not much is known about the compounds (especially saponins) in Shizhu ginseng.
A rapid, sensitive, and reliable ultra-high performance liquid chromatography coupled with MS/MS (UHPLC-MS/MS) method was developed to separate and identify saponins in Shizhu ginseng.
The separation was carried out on a Waters ACQUITY UPLC BEH C18 column (100 mm × 2.1 mm, 1.7 μm) with acetonitrile and 0.1% formic acid aqueous solution as the mobile phase under a gradient elution at 40°C. The detection was performed on a Micromass Quattro Micro API mass spectrometer equipped with electrospray ionization source in both positive and negative modes. Under the optimized conditions, a total of 31 saponins were identified or tentatively characterized by comparing retention time and MS data with related literatures and reference substances.
The developed UHPLC-MS/MS method was suitable for identifying and characterizing the chemical constituents in Shizhu ginseng, which provided a helpful chemical basis for further research on Shizhu ginseng.
在各种人参品种中，石柱参是中国特有的，主要分布在中国辽宁省宽甸满族自治县；然而，关于石柱参中的化合物（尤其是皂苷），人们了解得并不多。
建立了一种快速、灵敏且可靠的超高效液相色谱-串联质谱（UHPLC-MS/MS）方法，用于分离和鉴定石柱参中的皂苷。
在Waters ACQUITY UPLC BEH C18柱（100 mm×2.1 mm，1.7 μm）上进行分离，以乙腈和0.1%甲酸水溶液为流动相，在40°C下进行梯度洗脱。在配备电喷雾电离源的Micromass Quattro Micro API质谱仪上进行正、负离子模式检测。在优化条件下，通过将保留时间和质谱数据与相关文献及对照品进行比较，共鉴定或初步表征了31种皂苷。
所建立的UHPLC-MS/MS方法适用于鉴定和表征石柱参中的化学成分，为进一步研究石柱参提供了有益的化学依据。
