Rašković Aleksandar, Cvejić Jelena, Stilinović Nebojša, Goločorbin-Kon Svetlana, Vukmirović Saša, Mimica-Dukić Neda, Mikov Momir
Department of Pharmacology, Toxicology and Clinical Pharmacology, Faculty of Medicine, University of Novi Sad, Hajduk Veljkova 3, Novi Sad 21000, Serbia.
Laboratory for Pharmaceutical and Natural Products Analysis, Department of Pharmacy, Faculty of Medicine, University of Novi Sad, Hajduk Veljkova 3, Novi Sad 21000, Serbia.
Molecules. 2014 Mar 28;19(4):3869-82. doi: 10.3390/molecules19043869.
Herb-drug interactions are an important safety concern and this study was conducted regarding the interaction between the natural top-selling antidepressant remedy Hypericum perforatum (Hypericaceae) and conventional drugs. This study examined the influence of acute pretreatment with different extracts of Hypericum perforatum from Serbia on pentobarbital-induced sleeping time, impairment of motor coordination caused by diazepam and paracetamol pharmacokinetics in mice. Ethanolic extract, aqueous extract, infusion, tablet and capsule of Hypericum perforatum were used in this experiment. The profile of Hypericum perforatum extracts as well as paracetamol plasma concentration was determined using RP-HPLC analysis. By quantitative HPLC analysis of active principles, it has been proven that Hypericum perforatum ethanolic extract has the largest content of naphtodianthrones: hypericin (57.77 µg/mL) and pseudohypericin (155.38 µg/mL). Pretreatment with ethanolic extract of Hypericum perforatum potentiated the hypnotic effect of pentobarbital and impairment of motor coordination caused by diazepam to the greatest extent and also increased paracetamol plasma concentration in comparison to the control group. These results were in correlation with naphtodianthrone concentrations. The obtained results have shown a considerable influence of Hypericum perforatum on pentobarbital and diazepam pharmacodynamics and paracetamol pharmacokinetics.
草药-药物相互作用是一个重要的安全问题，本研究针对天然畅销抗抑郁药物贯叶连翘（金丝桃科）与传统药物之间的相互作用展开。本研究考察了塞尔维亚产贯叶连翘不同提取物急性预处理对小鼠戊巴比妥诱导睡眠时间、地西泮所致运动协调障碍以及对乙酰氨基酚药代动力学的影响。本实验使用了贯叶连翘的乙醇提取物、水提取物、浸剂、片剂和胶囊。采用反相高效液相色谱（RP-HPLC）分析法测定贯叶连翘提取物的概况以及对乙酰氨基酚的血浆浓度。通过对活性成分的定量HPLC分析，已证明贯叶连翘乙醇提取物中萘骈二蒽酮含量最高：金丝桃素（57.77μg/mL）和假金丝桃素（155.38μg/mL）。与对照组相比，贯叶连翘乙醇提取物预处理最大程度地增强了戊巴比妥的催眠作用以及地西泮所致的运动协调障碍，同时还提高了对乙酰氨基酚的血浆浓度。这些结果与萘骈二蒽酮浓度相关。所得结果表明贯叶连翘对戊巴比妥和地西泮的药效学以及对乙酰氨基酚的药代动力学有显著影响。
