Orčić Dejan Z, Mimica-Dukić Neda M, Francišković Marina M, Petrović Slobodan S, Jovin Emilija D
Department of Chemistry, Biochemistry and Environmental Protection, University of Novi Sad Faculty of Sciences, Trg Dositeja Obradovića 3, Novi Sad, Serbia.
Chem Cent J. 2011 Jun 25;5:34. doi: 10.1186/1752-153X-5-34.
The St John's Wort (Hypericum perforatum; Clusiaceae) has been used in traditional and modern medicine for a long time due to its high content of biologically active phenolics. The purpose of this work was to develop a method for their fractionation and identification, and to determine the most active antioxidant compounds in plant extract.
An LC-MS method which enables fast qualitative and semiquantitative analysis was developed. The composition determined is in agreement with the previous results, where 6 flavonoids, 4 naphthodianthrones and 4 phloroglucinols have been identified. Significant antioxidant activity was determined for most of the fractions by DPPH assay (the lowest IC50 of 0.52 μg/ml), NO scavenging (6.11 μg/ml), superoxide scavenging (1.86 μg/ml), lipid peroxidation (0.0079 μg/ml) and FRAP (the highest reduction capacity of 104 mg Fe equivalents/g) assays.
LC-MS technique has been successfully applied for a quick separation and identification of the major components of H. perforatum fractions. Majority of the fractions analyzed have expressed a very high antioxidative activity when compared to synthetic antioxidants. The antioxidant activity could be attributed to flavonoids and phenolic acids, while phloroglucinols and naphthodianthrones showed no significant activity. It is demonstrated that it is possible to obtain, by fractionation, H. perforatum preparations with significantly increased phloroglucinols-to-naphthodianthrones ratio (up to 95:5).
由于富含生物活性酚类物质，贯叶连翘（Hypericum perforatum；藤黄科）在传统医学和现代医学中已被使用很长时间。本研究的目的是开发一种对其进行分离和鉴定的方法，并确定植物提取物中最具活性的抗氧化化合物。
开发了一种能够进行快速定性和半定量分析的液相色谱 - 质谱联用（LC - MS）方法。所确定的成分与先前的结果一致，已鉴定出6种黄酮类化合物、4种萘并二蒽酮类化合物和4种间苯三酚类化合物。通过DPPH法（最低IC50为0.52μg/ml）、一氧化氮清除法（6.11μg/ml）、超氧阴离子清除法（1.86μg/ml）、脂质过氧化法（0.0079μg/ml）和铁离子还原抗氧化能力法（FRAP，最高还原能力为104mg铁当量/g）测定，发现大多数馏分具有显著的抗氧化活性。
LC - MS技术已成功应用于快速分离和鉴定贯叶连翘馏分中的主要成分。与合成抗氧化剂相比，大多数分析的馏分表现出非常高的抗氧化活性。抗氧化活性可能归因于黄酮类化合物和酚酸，而间苯三酚类化合物和萘并二蒽酮类化合物未显示出显著活性。结果表明，通过分馏有可能获得间苯三酚类化合物与萘并二蒽酮类化合物比例显著增加（高达95:5）的贯叶连翘制剂。
