用于牙周膜细胞递送的壳聚糖凝胶化的酶促控制。
Enzymatic control of chitosan gelation for delivery of periodontal ligament cells.
作者信息
Yan Xiang-Zhen, Nijhuis Arnold W G, van den Beucken Jeroen J J P, Both Sanne K, Jansen John A, Leeuwenburgh Sander C G, Yang Fang
机构信息
Department of Biomaterials, Radboud University Medical Center, 309 Dentistry, PO Box 9101, 6500 HB, Nijmegen, The Netherlands; Department of Periodontology, Shandong University, Jinan, 250012, Shandong, P. R. China.
出版信息
Macromol Biosci. 2014 Jul;14(7):1004-14. doi: 10.1002/mabi.201400040. Epub 2014 Mar 31.
The aim of this study is to optimize enzymatic control over gelation of chitosan-based hydrogels for the delivery of periodontal ligament cells (PDLCs). The results reveal that the gelation time, strength, and degradation rate of the chitosan hydrogels can be controlled precisely by variation of the urea and urease concentrations. PDLCs remain viable inside these hydrogels for up to 30 days. Cells released from the hydrogel upon degradation and collected after 3, 15, and 30 days are able to form colonies and osteogenically differentiate. In conclusion, the enzymatic control over the gelation of chitosan hydrogels offers options for the delivery of PDLCs.
本研究的目的是优化基于壳聚糖的水凝胶凝胶化的酶促控制，用于牙周膜细胞（PDLCs）的递送。结果表明，壳聚糖水凝胶的凝胶化时间、强度和降解速率可通过改变尿素和脲酶浓度来精确控制。PDLCs在这些水凝胶中可存活长达30天。在降解时从水凝胶中释放并在3天、15天和30天后收集的细胞能够形成集落并进行成骨分化。总之，壳聚糖水凝胶凝胶化的酶促控制为PDLCs的递送提供了选择。
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